Difference between revisions of "Part:BBa K3815011"

Revision as of 16:00, 20 October 2021

Part of the V-ATPaseB gene of Frankliniella occidentalis to synthesize dsRNA for RNAi ATPaseB gene of Frankliniella occidentalis to synthesize dsRNA for RNAi. To product dsRNA this sequence inserted in L4440 plasmid, and transformed into HT115(DE3).

purpose

We focused on feeding RNAi to kill these insects, based on previous studies showing that oral ingestion of dsRNA can induce RNAi-induced gene knockdown in Frankliniella occidentalis.
Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 452
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 320

@@ Line 2: / Line 2: @@
 __NOTOC__
 <partinfo>BBa_K3815011 short</partinfo>
-This is the Part of the V-ATPaseB gene of Frankliniella occidentalis to synthesize dsRNA for RNAi.
+ATPaseB gene of Frankliniella occidentalis to synthesize dsRNA for RNAi. To product dsRNA this sequence inserted in L4440 plasmid, and transformed into HT115(DE3).
-To product dsRNA this sequence inserted in L4440 plasmid, and transformed into HT115(DE3).
+<h3><font size="3"></font> purpose </h3>
+We focused on feeding RNAi to kill these insects, based on previous studies showing that oral ingestion of dsRNA can induce RNAi-induced gene knockdown in Frankliniella occidentalis.
+<br>
 <!-- Add more about the biology of this part here
 ===Usage and Biology===
@@ Line 15: / Line 13: @@
 <partinfo>BBa_K3815011 SequenceAndFeatures</partinfo>
+<h3><font size="3"> production and purification methods </font> </h3>
+<br>
 <!-- Uncomment this to enable Functional Parameter display