Difference between revisions of "Part:BBa K3760003"
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<partinfo>BBa_K3760003 short</partinfo>
 
<partinfo>BBa_K3760003 short</partinfo>
  
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A T7 promoter is added to 5' end of hACE2 gene &#65288;human angiotensin converting enzyme 2) This part is the template for RNA in vitro synthesis. The product will be the mRNA of hACE2 used in virification of RfxCas13d 's cleavage activity in virto.
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A T7 promoter is added to 5' end of hACE2 gene &#65288;human angiotensin converting enzyme 2) This part is the template for RNA in vitro synthesis. The product will be the mRNA of hACE2 used in verification of RfxCas13d 's cleavage activity in virto.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 15:58, 20 October 2021


T7 promoter + hACE2 target RNA coding sequence

A T7 promoter is added to 5' end of hACE2 gene （human angiotensin converting enzyme 2) This part is the template for RNA in vitro synthesis. The product will be the mRNA of hACE2 used in verification of RfxCas13d 's cleavage activity in virto.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 525
    Illegal BamHI site found at 1090
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2442
    Illegal SapI site found at 28
    Illegal SapI site found at 315