Difference between revisions of "Part:BBa K4013001"

 
 
(One intermediate revision by the same user not shown)
Line 7: Line 7:
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here
 
===Usage and Biology===
 
===Usage and Biology===
−
 
+
It contains promoter region and RBS, which we treated as a complete element for inserting engineering gene expression in Synechocystis 6803.
 
<!-- -->
 
<!-- -->
 
<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
Line 17: Line 17:
 
<partinfo>BBa_K4013001 parameters</partinfo>
 
<partinfo>BBa_K4013001 parameters</partinfo>
 
<!-- -->
 
<!-- -->
 +
Characterization: <br>
 +
These two native promoters are relatively weak in PCC 6803. We made this choice because excessive IAA would inhibit plant growth. For the terminator, we used a strong terminator called TrrnB.

Latest revision as of 15:34, 20 October 2021


P0452

P0452 is an endogenous promoter of cyanobacteria PCC6803 with an RBS, so CDS can be added directly through the restriction endonuclease digestion site.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Characterization:
These two native promoters are relatively weak in PCC 6803. We made this choice because excessive IAA would inhibit plant growth. For the terminator, we used a strong terminator called TrrnB.