Difference between revisions of "Part:BBa K3861024"

Revision as of 15:31, 20 October 2021

PlldR-sicP-sptP-Pep8

Plldr-sicP-spt-Pep8 Composite part consisting of BBa_K3861000 and BBa_K3861010. This way, the Pep8 expression is coupled to the enviromental lactate concentration mediated by PlldR. Further, due to the SPI T3SS secretion signal sptP and its chaperone sicP, Pep8 can be exported via the SPI T3SS.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal PstI site found at 519
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 78
Illegal NheI site found at 101
Illegal PstI site found at 519
21
COMPATIBLE WITH RFC[21]
23
INCOMPATIBLE WITH RFC[23]
Illegal PstI site found at 519
25
INCOMPATIBLE WITH RFC[25]
Illegal PstI site found at 519
1000
COMPATIBLE WITH RFC[1000]

@@ Line 5: / Line 5: @@
 Plldr-sicP-spt-Pep8
 <html>
-Composite part consisting of <a href="https://parts.igem.org/Part:BBa_K3861024?action=edit">BBa_K3861024</a> and <a href="https://parts.igem.org/Part:BBa_K3861010?action=edit">BBa_K3861010</a>. This way, the Pep8 expression is coupled to the enviromental lactate concentration mediated by PlldR. Further, due to the SPI T3SS secretion signal sptP and its chaperone sicP, Pep8 can be exported via the SPI T3SS.
+Composite part consisting of <a href="https://parts.igem.org/Part:BBa_K3861000?action=edit">BBa_K3861000</a> and <a href="https://parts.igem.org/Part:BBa_K3861010?action=edit">BBa_K3861010</a>. This way, the Pep8 expression is coupled to the enviromental lactate concentration mediated by PlldR. Further, due to the SPI T3SS secretion signal sptP and its chaperone sicP, Pep8 can be exported via the SPI T3SS.
 </html>