Difference between revisions of "Part:BBa K3739082"

Revision as of 15:25, 20 October 2021

Aly01-his-hutH-FT

Histidine ammonia-lyase with a his-tag is involved in catalyzing the reaction of changing L-histidine into trans-urocanate. Aly01 is a signal peptile. Direct fluorescent labeling of tetracysteine-motif-tagged (FlAsH tag, FT in short) secretory proteins with the biarsenical compound FlAsH is possible in situ without the need to recover the cell-free supernatant.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 237
Illegal NgoMIV site found at 673
Illegal NgoMIV site found at 1408
Illegal NgoMIV site found at 1644
Illegal AgeI site found at 73
Illegal AgeI site found at 310
Illegal AgeI site found at 1137
Illegal AgeI site found at 1633
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K3739082 short</partinfo>
-Histidine ammonia-lyase with a his-tag is involved in catalyzing the reaction of changing L-histidine into trans-urocanate. Aly01 is a signal peptile. Direct fluorescent labeling of tetracysteine-motif-tagged(FlAsH tag, FT in short) secretory proteins with the biarsenical compound FlAsH is possible in situ without the need to recover the cell-free supernatant.
+Histidine ammonia-lyase with a his-tag is involved in catalyzing the reaction of changing L-histidine into trans-urocanate. Aly01 is a signal peptile. Direct fluorescent labeling of tetracysteine-motif-tagged (FlAsH tag, FT in short) secretory proteins with the biarsenical compound FlAsH is possible in situ without the need to recover the cell-free supernatant.
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