Difference between revisions of "Part:BBa K3877005:Design"

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===Design Notes===
 
===Design Notes===
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We optimized the codon for the E. coli host. The fragment was then used to insert into the pET-30a vector through double digestion (NdeI-HindIII).
 
We optimized the codon for the E. coli host. The fragment was then used to insert into the pET-30a vector through double digestion (NdeI-HindIII).
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Latest revision as of 15:19, 20 October 2021


Tandem Algicidal Peptide SUMO His Tag Expression


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 545
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 319
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We optimized the codon for the E. coli host. The fragment was then used to insert into the pET-30a vector through double digestion (NdeI-HindIII).


Source

The protein-coding region was synthesized.

Use double digestion to reconstruct the expression vector.


References

Chen, X., Zaro, J. L., & Shen, W. C. (2013). Fusion protein linkers: property, design and functionality. Adv Drug Deliv Rev, 65(10), 1357-1369. https://doi.org/10.1016/j.addr.2012.09.039

Lee, J. K., & Park, Y. (2020). All d-Lysine Analogues of the Antimicrobial Peptide HPA3NT3-A2 Increased Serum Stability and without Drug Resistance. Int J Mol Sci, 21(16). https://doi.org/10.3390/ijms21165632

https://www.merckmillipore.com/CN/zh/product/pET-30a+-DNA-Novagen,EMD_BIO-69909