Difference between revisions of "Part:BBa K3829005"
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<br/><b>Fig.1</b> The construction of the gene circuit with Signal peptide (SS).
 
<br/><b>Fig.1</b> The construction of the gene circuit with Signal peptide (SS).
 
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<img src="https://2021.igem.org/wiki/images/c/c5/T--IvyMaker-China--Lab-12.jpg" style = "width:80%;">
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<br/><b>Fig.2</b> Representative images of the distribution of yeGPF.
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K3829005 Sequence And Features</partinfo>
  
  

Revision as of 15:08, 20 October 2021


Signal peptide-SS The signal peptide （SS） on the N terminus can lead the protein out of the cell.

Characterization

In our experiment, signal peptide (SS) was used. SS was a short peptide that could secrete proteins outside the cell. We constructed different gene circuits as shown in Figure 1 to verify the function of SS. The results showed that When SS was not present, fluorescence diffused in the cell (positive control). When SS was present (4609 and 5105), the fluorescence was displayed on the cell membrane, indicating that SS could secrete proteins outside the cell.


Fig.1 The construction of the gene circuit with Signal peptide (SS).

Fig.2 Representative images of the distribution of yeGPF.
Sequence and Features BBa_K3829005 Sequence And Features