Difference between revisions of "Part:BBa K4015006"
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<partinfo>BBa_K4015006 short</partinfo> | <partinfo>BBa_K4015006 short</partinfo> | ||
| − | Lcp-HlyA is composed of two basic parts, | + | Lcp-HlyA is composed of two basic parts, Lcp1VH2 and HlyA. HlyA and Lcp are connected by Gibson assembly in order to allow Lcp to be secreted outside the cell through HlyA. HlyA's C-terminal is fused with Lcp1VH2. |
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
===Usage and Biology=== | ===Usage and Biology=== | ||
| + | From the literature, we know that Lcp1VH2 possesses water solubility (andler, et al, 2019). Also, our experiments tell us that lcp is extremely difficult to purify. Therefore, we tried to use signal peptide and secretion system to cooperate to secrete the effective protein out of the cell. | ||
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Revision as of 14:58, 20 October 2021
Lcp-HlyA
Lcp-HlyA is composed of two basic parts, Lcp1VH2 and HlyA. HlyA and Lcp are connected by Gibson assembly in order to allow Lcp to be secreted outside the cell through HlyA. HlyA's C-terminal is fused with Lcp1VH2.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]