Difference between revisions of "Part:BBa K3888005:Experience"
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===Applications of BBa_K3888005=== | ===Applications of BBa_K3888005=== | ||
− | + | In order to achieve gene knockout, we used pK18mobsacB-Gm plasmid as a knockout vector. In addition, in order to achieve knockout, we selected about 1000bp sequences in the upstream and downstream of UppE and HupS respectively for design, amplified from the Rhodopseudomonas palustris CGA009 by PCR, and constructed knockout plasmids by the Gibson method (see the Design section for principles) ). The results of plasmid design and construction are as follows: | |
+ | [[File:UppE experiments.png]]<br> | ||
===User Reviews=== | ===User Reviews=== | ||
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Revision as of 14:32, 20 October 2021
Applications of BBa_K3888005
In order to achieve gene knockout, we used pK18mobsacB-Gm plasmid as a knockout vector. In addition, in order to achieve knockout, we selected about 1000bp sequences in the upstream and downstream of UppE and HupS respectively for design, amplified from the Rhodopseudomonas palustris CGA009 by PCR, and constructed knockout plasmids by the Gibson method (see the Design section for principles) ). The results of plasmid design and construction are as follows:
User Reviews
UNIQ572e0f5f866d9f47-partinfo-00000000-QINU UNIQ572e0f5f866d9f47-partinfo-00000001-QINU