Difference between revisions of "Part:BBa K3874004:Design"

 
(Design Notes)
 
Line 7: Line 7:
  
 
===Design Notes===
 
===Design Notes===
lactoferrin with EGFP  
+
This part is a fusion protein contain both lactoferrin and EGFP.
 
+
In order to make it express properly, we change some of the emzymtic site according to iGEM standard.
 
+
We also delete the stop codon of original part who express lactoferrin only and add an EGFP gene.
  
 
===Source===
 
===Source===

Latest revision as of 14:20, 20 October 2021


lactoferrin with EGFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part is a fusion protein contain both lactoferrin and EGFP. In order to make it express properly, we change some of the emzymtic site according to iGEM standard. We also delete the stop codon of original part who express lactoferrin only and add an EGFP gene.

Source

Cloning experiment

References