Difference between revisions of "Part:BBa K3747003:Experience"

 
 
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This experience page is provided so that any user may enter their experience using this part.<BR>Please enter
 
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how you used this part and how it worked out.
 
  
 
===Applications of BBa_K3747003===
 
===Applications of BBa_K3747003===
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https://2021.igem.org/wiki/images/f/fb/T--Wageningen_UR--MMO_figure_7.png
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SDS-page of particulate methane monooxygenase and scaffold. pMMO mimic as described in Kim et al 2019, expressed in <i>E. coli </i> BL21 strain with pET-28(+)-pMMO-m1 plasmid. The lanes harbor left to right (1) PageRuler Prestained Protein Ladder product #26616. (2) Product after Sonication. (3) Product after filtered. (4) Product after His-tag purification. The expected product size is 54 kDA in all cases and is annotated with the white asterisk in the figure.
  
 
===User Reviews===
 
===User Reviews===

Latest revision as of 13:57, 20 October 2021


Applications of BBa_K3747003

T--Wageningen_UR--MMO_figure_7.png

SDS-page of particulate methane monooxygenase and scaffold. pMMO mimic as described in Kim et al 2019, expressed in E. coli BL21 strain with pET-28(+)-pMMO-m1 plasmid. The lanes harbor left to right (1) PageRuler Prestained Protein Ladder product #26616. (2) Product after Sonication. (3) Product after filtered. (4) Product after His-tag purification. The expected product size is 54 kDA in all cases and is annotated with the white asterisk in the figure.

User Reviews

UNIQ6d3d4b1ef7e24569-partinfo-00000000-QINU UNIQ6d3d4b1ef7e24569-partinfo-00000001-QINU