Difference between revisions of "Part:BBa K3885111"
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In our experiment, we enhanced the expression of σ28 by adding UTR1 gene fragment before σ28 gene on the constructed plasmid. This part acts as a strong promoter. It can be combined with other genes to increase its expression. <br> | In our experiment, we enhanced the expression of σ28 by adding UTR1 gene fragment before σ28 gene on the constructed plasmid. This part acts as a strong promoter. It can be combined with other genes to increase its expression. <br> | ||
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<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
Revision as of 13:32, 20 October 2021
UTR1
UTR(Untranslated region (UTR) is the untranslated part at the 5' or 3' end of mature mRNA molecule, which generally plays an important role in mRNA transport, stability and translation regulation. The UTR1 sequence includes the T7 gene 10 leader RNA, which contains a strong ribosome binding site.
Usage and Biology
In our experiment, we enhanced the expression of σ28 by adding UTR1 gene fragment before σ28 gene on the constructed plasmid. This part acts as a strong promoter. It can be combined with other genes to increase its expression.
![](https://static.igem.org/mediawiki/parts/d/d7/Utr12.png")
Figure 1: The sequence of UTR1.
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Unknown
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]