Difference between revisions of "Part:BBa K3726058"

Latest revision as of 13:27, 20 October 2021

Lv2_BOH1_ABC

This is a composite part that corresponds with a level 2 MoClo part. It has three transcriptional units for the expression of the Lv1 parts “BBa_K3726044” BOH1_A_LV1, “BBa_K3726045” BOH1_B_LV1 and “BBa_K3726046” BOH1_C_LV1.

This level 2 part has as a function expressing the necessary enzymes for butanol biosynthesis through an artificial pathway. This part has been assembled following the marburg collection standard, then the transcriptional units are flanked by two homology regions “BBa_K3726104” 5CON1(H)_NS1(mod)-up (PCC 11801) and “BBa_K3726105 ” 3CON5(H)_NS1(mod)-down (PCC 11801) that allows the double homologous recombination within the genome of PCC 11801.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal EcoRI site found at 1230
Illegal EcoRI site found at 1850
Illegal PstI site found at 18
Illegal PstI site found at 1755
Illegal PstI site found at 2003
Illegal PstI site found at 3988
Illegal PstI site found at 4237
Illegal PstI site found at 7832
12
INCOMPATIBLE WITH RFC[12]
Illegal EcoRI site found at 1230
Illegal EcoRI site found at 1850
Illegal NheI site found at 6540
Illegal NheI site found at 6563
Illegal NheI site found at 6838
Illegal NheI site found at 7066
Illegal PstI site found at 18
Illegal PstI site found at 1755
Illegal PstI site found at 2003
Illegal PstI site found at 3988
Illegal PstI site found at 4237
Illegal PstI site found at 7832
21
INCOMPATIBLE WITH RFC[21]
Illegal EcoRI site found at 1230
Illegal EcoRI site found at 1850
Illegal BglII site found at 8166
Illegal BglII site found at 8283
Illegal BglII site found at 10702
Illegal BamHI site found at 4320
Illegal BamHI site found at 4659
Illegal XhoI site found at 5568
Illegal XhoI site found at 9386
Illegal XhoI site found at 10229
23
INCOMPATIBLE WITH RFC[23]
Illegal EcoRI site found at 1230
Illegal EcoRI site found at 1850
Illegal PstI site found at 18
Illegal PstI site found at 1755
Illegal PstI site found at 2003
Illegal PstI site found at 3988
Illegal PstI site found at 4237
Illegal PstI site found at 7832
25
INCOMPATIBLE WITH RFC[25]
Illegal EcoRI site found at 1230
Illegal EcoRI site found at 1850
Illegal PstI site found at 18
Illegal PstI site found at 1755
Illegal PstI site found at 2003
Illegal PstI site found at 3988
Illegal PstI site found at 4237
Illegal PstI site found at 7832
Illegal NgoMIV site found at 1494
Illegal NgoMIV site found at 1594
Illegal NgoMIV site found at 1900
Illegal NgoMIV site found at 4915
Illegal AgeI site found at 594
Illegal AgeI site found at 2272
Illegal AgeI site found at 3443
Illegal AgeI site found at 4443
1000
COMPATIBLE WITH RFC[1000]

@@ Line 5: / Line 5: @@
 This is a composite part that corresponds with a level 2 MoClo part. It has three  transcriptional units for the expression of the Lv1 parts “BBa_K3726044” BOH1_A_LV1, “BBa_K3726045” BOH1_B_LV1 and  “BBa_K3726046” BOH1_C_LV1.
-This level 2 part has as a function expressing the necessary enzymes for butanol biosynthesis through an artificial pathway ( X. Liu, R. Miao, P. Lindberg y P. Lindblad, “Modular engineering for efficient photosynthetic biosynthesis of 1-butanol from CO2 in cyanobacteria").
+This level 2 part has as a function expressing the necessary enzymes for butanol biosynthesis through an artificial pathway.
 This part has been assembled following the marburg collection standard, then the transcriptional units are flanked by  two homology regions “BBa_K3726104” 5CON1(H)_NS1(mod)-up (PCC 11801) and “BBa_K3726105 ” 3CON5(H)_NS1(mod)-down (PCC 11801) that allows the double homologous recombination within the genome of PCC 11801.