Difference between revisions of "Part:BBa K3734021"
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<p style="width: 80%;text-align:center;font-size: .9rem; margin: -1rem auto 1rem auto; color: #888;">Fig.2 Under Laser confocal microscopy, fluorescence of mCherry expression downstream of Tet-Off system</p> | <p style="width: 80%;text-align:center;font-size: .9rem; margin: -1rem auto 1rem auto; color: #888;">Fig.2 Under Laser confocal microscopy, fluorescence of mCherry expression downstream of Tet-Off system</p> | ||
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Latest revision as of 13:23, 20 October 2021
mCherry
The mCherry is a kind of fluorescin, whose excitation maximum is 587 nm.
mCherry
mCherry is a monomer red fluorescent protein. Experiments proved that when it is combined with exogenous protein on both C side and N side, the activity of fluorescent and the function of combined proteinare not effected and it can be used together with multi types of fluorescent proteins. Due to the phosphorylation pathway is relatively long, we have added mCherry at the bottom as report gene to prove that our pathway works and is regulated by insulin.
1.Pattern diagram
Fig.1 The model diagram of TRE-mCherry-miR21
2.Experiment
2.1 Method
Phosphated ELK will activate the expression of downstream report gene mCherry after the combination of TRE and TetR. We used lase confocal microscope to observe the expression.
2.2 Result
mCherry can be expressed and tested successfully
Fig.2 Under Laser confocal microscopy, fluorescence of mCherry expression downstream of Tet-Off system
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]