Difference between revisions of "Part:BBa K3734021"

 
 
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<partinfo>BBa_K3734021 short</partinfo>
 
<partinfo>BBa_K3734021 short</partinfo>
  
 
The mCherry is a kind of fluorescin, whose excitation maximum is 587 nm.
 
The mCherry is a kind of fluorescin, whose excitation maximum is 587 nm.
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                mCherry
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                <p>mCherry is a monomer red fluorescent protein. Experiments proved that when it is combined with exogenous protein on both C side and N side, the activity of fluorescent and the function of combined proteinare not effected and it can be
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                    used together with multi types of fluorescent proteins. Due to the phosphorylation pathway is relatively long, we have added mCherry at the bottom as report gene to prove that our pathway works and is regulated by insulin.
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                <!--put text here, <p>content</p>-->
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                1.Pattern diagram
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                    <img width="400px" src="https://2021.igem.org/wiki/images/1/13/T--CSU_CHINA--tupian20.png"></p>
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                <p style="width: 80%;text-align:center;font-size: .9rem; margin: -1rem auto 1rem auto; color: #888;">Fig.1 The model diagram of TRE-mCherry-miR21</p>
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                2.Experiment
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                2.1 Method
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                <p>Phosphated ELK will activate the expression of downstream report gene mCherry after the combination of TRE and TetR. We used lase confocal microscope to observe the expression.
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                2.2 Result
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                <p>mCherry can be expressed and tested successfully
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                <p style="text-align: center;">
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                    <img width="400px" src="https://2021.igem.org/wiki/images/5/54/T--CSU_CHINA--tupian14.png"></p>
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                <br>
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                <p style="width: 80%;text-align:center;font-size: .9rem; margin: -1rem auto 1rem auto; color: #888;">Fig.2 Under Laser confocal microscopy, fluorescence of mCherry expression downstream of Tet-Off system</p>
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here
 
===Usage and Biology===
 
===Usage and Biology===

Latest revision as of 13:23, 20 October 2021

mCherry

The mCherry is a kind of fluorescin, whose excitation maximum is 587 nm.

mCherry

mCherry is a monomer red fluorescent protein. Experiments proved that when it is combined with exogenous protein on both C side and N side, the activity of fluorescent and the function of combined proteinare not effected and it can be used together with multi types of fluorescent proteins. Due to the phosphorylation pathway is relatively long, we have added mCherry at the bottom as report gene to prove that our pathway works and is regulated by insulin.

1.Pattern diagram

Fig.1 The model diagram of TRE-mCherry-miR21

2.Experiment

2.1 Method

Phosphated ELK will activate the expression of downstream report gene mCherry after the combination of TRE and TetR. We used lase confocal microscope to observe the expression.

2.2 Result

mCherry can be expressed and tested successfully


Fig.2 Under Laser confocal microscopy, fluorescence of mCherry expression downstream of Tet-Off system

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]