Difference between revisions of "Part:BBa K4035002"
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<partinfo>BBa_K4035002 short</partinfo> | <partinfo>BBa_K4035002 short</partinfo> | ||
| − | This protein is made of two yeast copper metallotionein protein, CUP1 ( | + | This protein is made of two yeast copper metallotionein protein, CUP1 ([https://parts.igem.org/Part:BBa_M45090 BBa_M45090]), linked together by a flexible linker made of three times the GGGGS amino acid sequence. |
| − | + | ==Usage and Biology== | |
| − | Copper metallotionein CUP1 (BBa_M45090) is a protein responsible for copper binding protein in the yeast Saccharomyces cerevisiae. In order to increase the copper retrieval efficiency, two copies of CUP1 were linked together and expressed at the outter surface of S. cerevisiae (BBa_K4035009). The GGGGS is a common flexible linker and was inserted 3 times in order to allow distances between the two copies of CUP1 and avoid interaction. | + | Copper metallotionein CUP1 ([https://parts.igem.org/Part:BBa_M45090 BBa_M45090]) is a protein responsible for copper binding protein in the yeast Saccharomyces cerevisiae. In order to increase the copper retrieval efficiency, two copies of CUP1 were linked together and expressed at the outter surface of S. cerevisiae ([https://parts.igem.org/Part:BBa_K4035009 BBa_K4035009]). The GGGGS is a common flexible linker and was inserted 3 times in order to allow distances between the two copies of CUP1 and avoid interaction. |
| − | + | ==Sequence and Features== | |
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<partinfo>BBa_K4035002 SequenceAndFeatures</partinfo> | <partinfo>BBa_K4035002 SequenceAndFeatures</partinfo> | ||
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| + | ==Characterization== | ||
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Revision as of 12:58, 20 October 2021
Dimerization of the copper metallothionein 1 : CUP1-(GGGGS)3-CUP1
This protein is made of two yeast copper metallotionein protein, CUP1 (BBa_M45090), linked together by a flexible linker made of three times the GGGGS amino acid sequence.
Usage and Biology
Copper metallotionein CUP1 (BBa_M45090) is a protein responsible for copper binding protein in the yeast Saccharomyces cerevisiae. In order to increase the copper retrieval efficiency, two copies of CUP1 were linked together and expressed at the outter surface of S. cerevisiae (BBa_K4035009). The GGGGS is a common flexible linker and was inserted 3 times in order to allow distances between the two copies of CUP1 and avoid interaction.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 190
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]