Difference between revisions of "Part:BBa K3815015"
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This is an engineered derivative of wildtype ssrA tag from <i>Escherichia coli</i>, acquired by mutagenizing the WT tag with mixed primers. Compared to the WT, three C-terminal amino acids LAA are replaced to LGA. To quantify its efficiency of protein degradation, ... of <i>E.coli</i> overnight culture bearing a plasmid expressing GFP-ssrA tag fusion protein was measured by Qubit, and then compared to the WT and other mutants. | This is an engineered derivative of wildtype ssrA tag from <i>Escherichia coli</i>, acquired by mutagenizing the WT tag with mixed primers. Compared to the WT, three C-terminal amino acids LAA are replaced to LGA. To quantify its efficiency of protein degradation, ... of <i>E.coli</i> overnight culture bearing a plasmid expressing GFP-ssrA tag fusion protein was measured by Qubit, and then compared to the WT and other mutants. | ||
| + | ==Usage and Biology== | ||
| + | <br> | ||
| + | <br> | ||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
Revision as of 12:53, 20 October 2021
AANDENYALGA. mutant SsrA degradation tag
This is an engineered derivative of wildtype ssrA tag from Escherichia coli, acquired by mutagenizing the WT tag with mixed primers. Compared to the WT, three C-terminal amino acids LAA are replaced to LGA. To quantify its efficiency of protein degradation, ... of E.coli overnight culture bearing a plasmid expressing GFP-ssrA tag fusion protein was measured by Qubit, and then compared to the WT and other mutants.
Usage and Biology
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]