Difference between revisions of "Part:BBa K3885126"

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	<p style="text-align: center:">Figure 2. Schematic of gene circuits and kinetics of tetR inhibition in the Cell-Free system.<br>		<p style="text-align: center:">Figure 2. Schematic of gene circuits and kinetics of tetR inhibition in the Cell-Free system.<br>
	(A) The gene circuits contains three parts, sigma 28 activates promoter P28 to express tet repressor with ssrA tag, which inhibits the expression of deGFP. <br>		(A) The gene circuits contains three parts, sigma 28 activates promoter P28 to express tet repressor with ssrA tag, which inhibits the expression of deGFP. <br>

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Revision as of 12:45, 20 October 2021

tetR-ssrA

SsrA is a degradation tag, added at the 3 end of tetR that degrades the repressor protein tetR

Usage and Biology

To ensure the normal operation of the Cell-Free system, an adjustable protein degradation mechanism is crucial. It is degraded by ClpXPAAA+ protease in Escherichia coli . The protein must contain a degradation tag in order to be recognized by our cell-free system .
As the improvement of the 10_Heidelberg registered part (BBa_K337028: tet Repressor) , the degradation tag ssrA is added to the 3' end of the tetR (Figure 1) , hence the newly registered partBBa_K3885126.

Characterization

TetR with ssrA can be degraded by ClpXP, so that the flourescence intensity increased as a result of the inhibition released from tet Repressor.
Therefore, we gave the tetR a new function and improved this part. Sequence and Features