Difference between revisions of "Part:BBa K3995003"

Latest revision as of 12:39, 20 October 2021

P7_AtzR

Profile

Name: P7_AtzR

Base Pairs: 1142 bp

Origin: Synthetic

Properties: A coding sequence for atzR

Usage and Biology

P7_AtzR is a coding sequence for expressing atzR. Cyanuric acid combination with AtzR can activate amilGFP expression to show the signs of fluorescence. The content of cyanuric acid is detected by detecting the fluorescence concentration. So P7_AtzR should be used with Pprovoin5_amilGFP.

Figure1. Figure 1 Genetic Design of our Sensor.

Construct design

The atzR is under T7 promoter. The P7_atzR is inserted in the pUC57 mini vector (Figure 3).

Figure 2. P7_AtzR box..

Figure 3. Schematic maps of P7_AtzR (sensor plasmid).

BBa_K3995000

Name: P7

Base Pairs: 19bp

Origin: T7 phage

Properties: A promoter for initiation of the transcription.

Usage and Biology

The promoter is recognized for binding and initiation of the transcription. Placed before a gene, it promotes its transcription.

BBa_K3995001

Name: AtzR

Base Pairs: 969bp

Origin: Pseudomonas sp.

Properties: A protein binding cyanuric acid

Usage and Biology

BBa_K3995001 is a coding sequence of atzR. Cyanuric acid combining with atzR can activates Pprovoin5 promoter. So atzR can act as a sensor.

BBa_K4030002

Name: TT

Base Pairs: 140bp

Origin: Escherichia coli

Properties: Transcription terminator

Usage and Biology

It is an transcription terminator derived from the E.coli rrB rRNA operon

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal EcoRI site found at 412
Illegal EcoRI site found at 955
12
INCOMPATIBLE WITH RFC[12]
Illegal EcoRI site found at 412
Illegal EcoRI site found at 955
21
INCOMPATIBLE WITH RFC[21]
Illegal EcoRI site found at 412
Illegal EcoRI site found at 955
23
INCOMPATIBLE WITH RFC[23]
Illegal EcoRI site found at 412
Illegal EcoRI site found at 955
25
INCOMPATIBLE WITH RFC[25]
Illegal EcoRI site found at 412
Illegal EcoRI site found at 955
Illegal AgeI site found at 802
1000
COMPATIBLE WITH RFC[1000]

@@ Line 4: / Line 4: @@
 P7_AtzR
+=== Profile ===
+=== Name: P7_AtzR ===
+==== Base Pairs: 1142 bp ====
+==== Origin: Synthetic ====
+==== Properties: A coding sequence for atzR ====
+=== Usage and Biology ===
+P7_AtzR is a coding sequence for expressing atzR. Cyanuric acid combination with AtzR can activate amilGFP expression to show the signs of fluorescence. The content of cyanuric acid is detected by detecting the fluorescence concentration. So P7_AtzR should be used with Pprovoin5_amilGFP.
+[[File:T--ECNUAS--BBa K3995005-Figure1.png|500px|thumb|center|Figure1. Figure 1 Genetic Design of our Sensor.]]
-<!-- Add more about the biology of this part here
+=== Construct design ===
-===Usage and Biology===
+The atzR is under T7 promoter. The P7_atzR is inserted in the pUC57 mini vector (Figure 3).
+[[File:T--ECNUAS--BBa K3995003-Figure2.png|500px|thumb|center|Figure 2. P7_AtzR box..]]
+[[File:T--ECNUAS--BBa K3995005-Figure3.png|500px|thumb|center|Figure 3. Schematic maps of P7_AtzR (sensor plasmid).]]
+== BBa_K3995000 ==
+Name: P7
+Base Pairs: 19bp
+Origin: T7 phage
+Properties: A promoter for initiation of the transcription.
+==== Usage and Biology ====
+The promoter is recognized for binding and initiation of the transcription. Placed before a gene, it promotes its transcription.
+== BBa_K3995001==
+Name: AtzR
+Base Pairs: 969bp
+Origin: Pseudomonas sp.
+Properties: A protein binding cyanuric acid
+==== Usage and Biology ====
+BBa_K3995001 is a coding sequence of atzR. Cyanuric acid combining with atzR can activates Pprovoin5 promoter. So atzR can act as a sensor.
+== BBa_K4030002 ==
+Name: TT
+Base Pairs: 140bp
+Origin: Escherichia coli
+Properties: Transcription terminator
+==== Usage and Biology ====
+It is an transcription terminator derived from the E.coli rrB rRNA operon
 <!-- -->