Difference between revisions of "Part:BBa K3805999:Design"
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+ | __NOTOC__ | ||
+ | <partinfo>BBa_K3805999 short</partinfo> | ||
+ | <partinfo>BBa_K3805999 SequenceAndFeatures</partinfo> | ||
+ | |||
+ | ===Designed Notes=== | ||
+ | |||
+ | To ensure the specificity of binding, 19bp sequence on the T7 promoter was selected as sgRNA. We design the special sgRNA on website http://crispr.dfci.harvard.edu/SSC/ which is owned by Harvard. | ||
+ | |||
+ | ===Source=== | ||
+ | |||
+ | We expect to synthesize this fragment. |
Latest revision as of 12:30, 20 October 2021
sgRNA bind to T7 promoter
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Designed Notes
To ensure the specificity of binding, 19bp sequence on the T7 promoter was selected as sgRNA. We design the special sgRNA on website http://crispr.dfci.harvard.edu/SSC/ which is owned by Harvard.
Source
We expect to synthesize this fragment.