Difference between revisions of "Part:BBa K3815015"

Revision as of 12:27, 20 October 2021

AANDENYALGA. mutant SsrA degradation tag

This is an engineered derivative of wildtype ssrA tag from Escherichia coli, acquired by mutagenizing the WT tag with random-base primers. Compared to the WT, three C-terminal amino acids LAA are replaced to LGA. To quantify its efficiency of protein degradation, ... of E.coli overnight culture bearing a plasmid expressing GFP-ssrA tag fusion protein was measured by Qubit, and then compared to the WT and other mutants.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 <partinfo>BBa_K3815015 short</partinfo>
-This is an engineered derivative of wildtype ssrA tag from <i>Escherichia coli</i>, acquired by mutagenizing the WT tag with random-base primers. Compared to the WT, three C-terminal amino acids LAA were replaced to LGA.
+This is an engineered derivative of wildtype ssrA tag from <i>Escherichia coli</i>, acquired by mutagenizing the WT tag with random-base primers. Compared to the WT, three C-terminal amino acids LAA are replaced to LGA. To quantify its efficiency of protein degradation, ... of <i>E.coli</i> overnight culture bearing a plasmid expressing GFP-ssrA tag fusion protein was measured by Qubit, and then compared to the WT and other mutants.
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