Difference between revisions of "Part:BBa K3888004"
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<partinfo>BBa_K3888004 short</partinfo> | <partinfo>BBa_K3888004 short</partinfo> | ||
− | HoxK1 coding gene with E. coli signal sequence | + | HoxK1 coding gene with E. coli signal sequence<br> |
This basic part is used to achieve heterologous expression of small subunit of oxygen-tolerant Hydrogenovibrio marinus [NiFe]-hydrogenase in Escherichia coli. HoxK1 encodes the small subunit of hydrogenase mentioned above, and together with HoxG1(BBa_K3888003), to form composite part Core Gene HoxG1 and HoxK1(BBa_K3888011). | This basic part is used to achieve heterologous expression of small subunit of oxygen-tolerant Hydrogenovibrio marinus [NiFe]-hydrogenase in Escherichia coli. HoxK1 encodes the small subunit of hydrogenase mentioned above, and together with HoxG1(BBa_K3888003), to form composite part Core Gene HoxG1 and HoxK1(BBa_K3888011). | ||
[[File:pET28-pTac-lacO-hydrogenase .png]] | [[File:pET28-pTac-lacO-hydrogenase .png]] |
Revision as of 12:00, 20 October 2021
HoxK1
HoxK1 coding gene with E. coli signal sequence
This basic part is used to achieve heterologous expression of small subunit of oxygen-tolerant Hydrogenovibrio marinus [NiFe]-hydrogenase in Escherichia coli. HoxK1 encodes the small subunit of hydrogenase mentioned above, and together with HoxG1(BBa_K3888003), to form composite part Core Gene HoxG1 and HoxK1(BBa_K3888011).
Figure 1: The Oxygen-tolerant Hydrogenase introduction plasmid constructed, which has a length of 8261bp.
HoxK1 encodes the small subunit of oxygen-tolerant hydrogenase, and it's controlled by a Lac operator.
Sequence and Features
HoxK1 has a length of 966bp, which is located from 7137bp to 8102bp on the plasmid diagram showed above.
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 940
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]