Difference between revisions of "Part:BBa K3888003:Design"
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===Figure 1: The Oxygen-tolerant Hydrogenase introduction plasmid constructed, which has a length of 8261bp.=== | ===Figure 1: The Oxygen-tolerant Hydrogenase introduction plasmid constructed, which has a length of 8261bp.=== | ||
| − | HoxG1 encodes the large subunit of oxygen-tolerant hydrogenase, and it's controlled by a Lac operator | + | HoxG1 encodes the large subunit of oxygen-tolerant hydrogenase, and it's controlled by a Lac operator. |
<partinfo>BBa_K3888003 SequenceAndFeatures</partinfo> | <partinfo>BBa_K3888003 SequenceAndFeatures</partinfo> | ||
Latest revision as of 10:28, 20 October 2021
HoxG1
Figure 1: The Oxygen-tolerant Hydrogenase introduction plasmid constructed, which has a length of 8261bp.
HoxG1 encodes the large subunit of oxygen-tolerant hydrogenase, and it's controlled by a Lac operator.
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 282
Illegal XbaI site found at 384
Illegal SpeI site found at 450 - 12INCOMPATIBLE WITH RFC[12]Illegal SpeI site found at 450
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 597
Illegal BamHI site found at 1512
Illegal XhoI site found at 1834 - 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 282
Illegal XbaI site found at 384
Illegal SpeI site found at 450 - 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 282
Illegal XbaI site found at 384
Illegal SpeI site found at 450
Illegal AgeI site found at 1728 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
Codon optimization for E. coli
Source
Hydrogenovibrio marinus MH-110 Genome
References
1. Kim, Jaoon YH, Byung Hoon Jo, and Hyung Joon Cha. "Production of biohydrogen by heterologous expression of oxygen-tolerant Hydrogenovibrio marinus [NiFe]-hydrogenase in Escherichia coli." Journal of biotechnology 155.3 (2011): 312-319.