Difference between revisions of "Part:BBa K4011002"
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Ma-Mut is an mutated protein tag derived from the protein secretion tag (Ma) of mating type alpha <i>Saccharomyces cerevisiae</i>. In yeast cells, proteins with the Ma tag will be secreted out of the cell. This year, we will use Ma-Mut to enable secretion of sfGFP-CBM3 and CBM3-NT2RepCT-CBM3 from yeast. This will allow for modification of our bacterial cellulose membrane produced by a co-culture of <i>Komagataeibacter</i> and yeast. We will construct composite parts Ma-sfGFP-CBM3 <partinfo>BBa_K4011010</partinfo> and Ma-CBM3-NT2RepCT-CBM3 <partinfo>BBa_K4011011</partinfo> with Ma_Mut.  
 
Ma-Mut is an mutated protein tag derived from the protein secretion tag (Ma) of mating type alpha <i>Saccharomyces cerevisiae</i>. In yeast cells, proteins with the Ma tag will be secreted out of the cell. This year, we will use Ma-Mut to enable secretion of sfGFP-CBM3 and CBM3-NT2RepCT-CBM3 from yeast. This will allow for modification of our bacterial cellulose membrane produced by a co-culture of <i>Komagataeibacter</i> and yeast. We will construct composite parts Ma-sfGFP-CBM3 <partinfo>BBa_K4011010</partinfo> and Ma-CBM3-NT2RepCT-CBM3 <partinfo>BBa_K4011011</partinfo> with Ma_Mut.  
  
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===Usage and Biology===
 
===Usage and Biology===
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===Design Considerations===
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The following mutations are added in Ma_Mut: A9D, A20T, Q32H, F48S, and G62R.
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The last eight amino acids is a linker with the sequence EAEAEFGS.
  
 
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Revision as of 09:49, 20 October 2021


Ma-Mut

Ma-Mut is an mutated protein tag derived from the protein secretion tag (Ma) of mating type alpha Saccharomyces cerevisiae. In yeast cells, proteins with the Ma tag will be secreted out of the cell. This year, we will use Ma-Mut to enable secretion of sfGFP-CBM3 and CBM3-NT2RepCT-CBM3 from yeast. This will allow for modification of our bacterial cellulose membrane produced by a co-culture of Komagataeibacter and yeast. We will construct composite parts Ma-sfGFP-CBM3 BBa_K4011010 and Ma-CBM3-NT2RepCT-CBM3 BBa_K4011011 with Ma_Mut.

Usage and Biology

Design Considerations

The following mutations are added in Ma_Mut: A9D, A20T, Q32H, F48S, and G62R.

The last eight amino acids is a linker with the sequence EAEAEFGS.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]