Difference between revisions of "Part:BBa K792002"
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To use this tag, '''attached it directly to your proteins ''CDS'''''. | To use this tag, '''attached it directly to your proteins ''CDS'''''. | ||
+ | |||
+ | {| style="width:100%" | ||
+ | |- | ||
+ | | style="background: yellow; width:11%" rowspan="2"| '''See also: ''' | ||
+ | | style="background: #D8D8D8" | A Kozak sequence from α-factor mating pheromone (MFα1) <partinfo>K792001</partinfo> | ||
+ | |} | ||
+ | |||
+ | {| style="width:100%; background: #CEE3F6" | ||
+ | |- | ||
+ | |This parts was originally built to be used in [http://2012.igem.org/Team:Buenos_Aires iGEM BsAs 2012 project] to implement a ''cross-feeding'' regulatory system between two different yeast strains with specific auxotrophy. [http://2012.igem.org/Team:Buenos_Aires Visit our wiki] to read details about the designing process and implementation details of our system. Also feel free to contact us for any question regarding this part. | ||
+ | |[[Image:Igem.bsas.png | 100px]] | ||
+ | |} | ||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
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<partinfo>BBa_K792002 SequenceAndFeatures</partinfo> | <partinfo>BBa_K792002 SequenceAndFeatures</partinfo> | ||
+ | === <h2>Part Contributions</h2> === | ||
+ | === NUS Singapore 2021 === | ||
+ | '''Authors:''' | ||
+ | |||
+ | Tania Santosh Nair, Chew Chin Wei, Linus Tan | ||
+ | |||
+ | '''Contribution Summary:''' | ||
+ | |||
+ | Team NUS_Singapore 2021 has successfully expressed GFP using this part to quantify the performance. Characterization of part performance was also done using a GFP reporter. Check NUS Singapore 2021 wiki to view more information: https://2021.igem.org/Team:NUS_Singapore/Results | ||
+ | |||
+ | [[File:T--NUS Singapore--GFP plateimage.png|800px|thumb|center|Figure 1: Image of the media supernatant, resuspended cells and blank control media under gel visualizer blue light, media and supernatant are clearly fluorescent compared to the control media.]] | ||
+ | |||
+ | This part was placed at the N-terminus of a GFP reporter gene. The expression of the mFa-GFP fusion protein was controlled using a Gal1 inducible promoter (Figure 2). | ||
+ | |||
+ | [[File:T--NUS Singapore--NUS Singapore--pGmGFP-H.png|800px|thumb|center|Figure 2: mFa-GFP Assembly schematic of the construct used to characterize performance of this secretion tag.]] | ||
+ | |||
+ | GFP absorbance of the supernatant and cells of both induced and uninduced pGmFaGFP-H (BY4741) was recorded (figure 3) after 24 hrs of induction in galactose enriched yeast growth medium. Secretion efficacy of the GFP reporter gene was approximated to about 42%. | ||
+ | |||
+ | [[File:T--NUS Singapore--pGmGFP-H graph.png|800px|thumb|center|Figure 3: GFP absorbance of the supernatant and cells of both induced and uninduced pGmFaGFP-H (BY4741), after normalization to blank media. Induced cells secreted approximately 42% of the GFP, while uninduced cells produced and secreted significantly less GFP than induced cells.]] | ||
+ | |||
+ | <!-- [[File:|700px|thumb|center|]] <!-- --> | ||
+ | |||
+ | '''Additional Information''' | ||
+ | |||
+ | Please visit our results page for more information on the characterization data of this part here: https://2021.igem.org/Team:NUS_Singapore/Results | ||
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Latest revision as of 09:31, 20 October 2021
Secretion tag from yeast α-factor mating pheromone (MFα1)
This part is the secretion signal peptide from the yeast α-mating factor. This signal peptide directs the secretion of the produced protein, and therefore allows the exportation of it. This peptides are cleaved once the protein is in the lumen of the ER.
To use this tag, attached it directly to your proteins CDS.
See also: | A Kozak sequence from α-factor mating pheromone (MFα1) BBa_K792001 |
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Part Contributions
NUS Singapore 2021
Authors:
Tania Santosh Nair, Chew Chin Wei, Linus Tan
Contribution Summary:
Team NUS_Singapore 2021 has successfully expressed GFP using this part to quantify the performance. Characterization of part performance was also done using a GFP reporter. Check NUS Singapore 2021 wiki to view more information: https://2021.igem.org/Team:NUS_Singapore/Results
This part was placed at the N-terminus of a GFP reporter gene. The expression of the mFa-GFP fusion protein was controlled using a Gal1 inducible promoter (Figure 2).
GFP absorbance of the supernatant and cells of both induced and uninduced pGmFaGFP-H (BY4741) was recorded (figure 3) after 24 hrs of induction in galactose enriched yeast growth medium. Secretion efficacy of the GFP reporter gene was approximated to about 42%.
Additional Information
Please visit our results page for more information on the characterization data of this part here: https://2021.igem.org/Team:NUS_Singapore/Results