Difference between revisions of "Part:BBa K4032105"

Revision as of 09:00, 20 October 2021

lacI+RBS+DegP+double terminator

Contents:

・The lac promoter (https://parts.igem.org/Part:BBa_R0010)

・The RBS (https://parts.igem.org/Part:BBa_B0034)

・The gene of DegP protein.

・The double terminator (https://parts.igem.org/Part:BBa_B0015)

Usage and Biology

DegP is a type of heat shock protein in E.coli. Heat shock protein is a family of proteins whose expression is induced by SOS responses such as heat.

It is also a type of serine protease due to the presence of serine residues in the active center.

In particular, it recognizes and degrades peptides whose structure has been damaged by heat or misfolding.

Designs

The lac promoter , the RBS and the double terminator are added to BBa_K40320xx, forming this part.

The lac promoter , the RBS and the double terminator are used from BBa_J04450 (https://parts.igem.org/Part:BBa_J04450) .

This part was created by the In-Fusion method.

Sequence and Features