Difference between revisions of "Part:BBa K4032105"
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<partinfo>BBa_K4032105 short</partinfo>
 
<partinfo>BBa_K4032105 short</partinfo>
  
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This part contains sequences of DegP protein.
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Contents:
  
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This part is the addition of a lac promoter and RBS and double terminator to BBa_K40320xx. Lac promoter and RBS and double terminator were used as contained in BBa_J04450.
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・The lac promoter Part:BBa R0010 - parts.igem.org
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・The  RBS (Part:BBa B0034 - parts.igem.org)
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・The gene of DegP protein.
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・The double terminator Part:BBa B0015 - parts.igem.org
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===Usage and Biology===
  
 
DegP is a type of heat shock protein in E.coli. Heat shock protein is a family of proteins whose expression is induced by SOS responses such as heat.  
 
DegP is a type of heat shock protein in E.coli. Heat shock protein is a family of proteins whose expression is induced by SOS responses such as heat.  
 +
 
It is also a type of serine protease due to the presence of serine residues in the active center.
 
It is also a type of serine protease due to the presence of serine residues in the active center.
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In particular, it recognizes and degrades peptides whose structure has been damaged by heat or misfolding.
 
In particular, it recognizes and degrades peptides whose structure has been damaged by heat or misfolding.
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 +
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===Designs===
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The lac promoter , the RBS and the double terminator are added to BBa_K40320xx, forming this part.
 +
 +
The lac promoter , the RBS and the double terminator are used from BBa_J04450 (https://parts.igem.org/Part:BBa_J04450) .
  
 
This part was created by the In-Fusion method.
 
This part was created by the In-Fusion method.
 +
  
  

Revision as of 08:56, 20 October 2021


lacI+RBS+DegP+double terminator

Contents:

・The lac promoter Part:BBa R0010 - parts.igem.org

・The RBS (Part:BBa B0034 - parts.igem.org)

・The gene of DegP protein.

・The double terminator Part:BBa B0015 - parts.igem.org


Usage and Biology

DegP is a type of heat shock protein in E.coli. Heat shock protein is a family of proteins whose expression is induced by SOS responses such as heat.

It is also a type of serine protease due to the presence of serine residues in the active center.

In particular, it recognizes and degrades peptides whose structure has been damaged by heat or misfolding.


Designs

The lac promoter , the RBS and the double terminator are added to BBa_K40320xx, forming this part.

The lac promoter , the RBS and the double terminator are used from BBa_J04450 (https://parts.igem.org/Part:BBa_J04450) .

This part was created by the In-Fusion method.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1364
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1364
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1364
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1364
    Illegal AgeI site found at 2206
    Illegal AgeI site found at 2318
  • 1000
    COMPATIBLE WITH RFC[1000]