Difference between revisions of "Part:BBa K3887012"
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<partinfo>BBa_K3887012 short</partinfo> | <partinfo>BBa_K3887012 short</partinfo> | ||
| − | + | Single guide RNA sequence we designed for TnaA knockout in E.coli BL21(DE3). | |
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| + | Single guide RNA (sgRNA) is used during gene editing process. It is the sequences of RNA molecules that bind to the target location. It is made of crRNA and trcrRNA. During the editing process, tracrRNA first recognizes the Cas9 and binds to it. The Cas9 receives sequence information from the crRNA and moves to the target location to perform the cleaving of DNA. | ||
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Latest revision as of 08:45, 20 October 2021
sgRNA2
Single guide RNA sequence we designed for TnaA knockout in E.coli BL21(DE3).
Single guide RNA (sgRNA) is used during gene editing process. It is the sequences of RNA molecules that bind to the target location. It is made of crRNA and trcrRNA. During the editing process, tracrRNA first recognizes the Cas9 and binds to it. The Cas9 receives sequence information from the crRNA and moves to the target location to perform the cleaving of DNA.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]