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| __NOTOC__ | | __NOTOC__ |
| <partinfo>BBa_K3734006 short</partinfo> | | <partinfo>BBa_K3734006 short</partinfo> |
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| <head> | | <head> |
− | <meta charset="utf-8" /> | + | |
− | <meta name="viewport" content="width=device-width, initial-scale=1, shrink-to-fit=no" />
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− | <meta name="csu_china" content="csu_china" />
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− | <title>Team:csu_china</title>
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− | <!-- Favicon-->
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− | <link rel="icon" type="image/x-icon" href="assets/favicon.ico" />
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− | <!-- Font Awesome icons (free version)-->
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− | <script src="https://use.fontawesome.com/releases/v5.15.3/js/all.js" crossorigin="anonymous"></script>
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− | <!-- Google fonts-->
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− | <link href="https://fonts.googleapis.com/css?family=Roboto+Slab:400,100,300,700" rel="stylesheet" type="text/css" />
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− | <!-- Core theme CSS (includes Bootstrap)-->
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− | | + | |
− | <script src="http://code.jquery.com/jquery-latest.js"></script>
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− | <script src="https://2021.igem.org/wiki/index.php?title=Template:CSU_CHINA/JS/script&action=raw&ctype=text/javascript"></script>
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− | <script src="https://2021.igem.org/wiki/index.php?title=Template:CSU_CHINA/JS/scripts&action=raw&ctype=text/javascript"></script>
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| </head> | | </head> |
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| <body> | | <body> |
− | <style>
| + | <h2 class="pageContent-main__title"><!--put tile here, <h2>title</h2>, class="pageContent-main__title" means it is the main title--> |
− | .MathJax nobr>span.math>span {
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− | *,
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− | *:before,
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− | *:after {
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− | :root {
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− | --primary: #074E9F;
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− | --primary-transparent: rgba(7, 78, 159, 0.5);
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− | --blue: #6DBFFF;
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− | --yellow: #FFC93C;
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− | --dark: #0d2b4d;
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− | --title-color: #004290;
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− | /* background-color: */
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− | text-align: center;
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− | #collapse {
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− | #top_title {
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− | #collapse>.navbar-list {
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− | padding: .5rem;
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− | flex-direction: row;
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− | /* overflow: auto; */
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− | #collapse>.navbar-list>.navbar-list__item {
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− | font-weight: bold;
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− | padding: .3rem 1rem;
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− | /* flex: 1; */
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− | transition: all .3s;
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− | #collapse>.navbar-list>.navbar-list__item .navbar-list__menu {
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− | top: 2.2rem;
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− | border-radius: 5px;
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− | box-shadow: 0 3px 10px -2px rgba(0, 0, 0, 0.1);
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− | width: 10rem;
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− | left: calc(50% - 5rem);
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− | }
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− | #collapse>.navbar-list>.navbar-list__item .navbar-list__menuItem {
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− | padding: .5rem 1rem;
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− | cursor: pointer;
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− | #collapse>.navbar-list>.active,
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− | #collapse>.navbar-list>.navbar-list__item:hover {
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− | background-color: var(--yellow);
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− | color: var(--primary);
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− | #collapse>.navbar-list>.teamname {
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− | font-size: 1.5rem;
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− | color: var(--yellow);
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− | }
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− | #logo {
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− | width: 2.5rem;
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− | height: 2.5rem;
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− | }
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− | #home {
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− | width: 100%;
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− | min-height: 100vh;
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− | background-color: #ffffff;
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− | display: flex;
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− | flex-direction: column;
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− | }
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− |
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− | #home>.pageHead {
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− | height: 80vh;
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− | width: 100%;
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− | position: relative;
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− | background-position-y: top;
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− | background-position-x: center;
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− | background-size: cover;
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− | background-image: url('/static/images/lab.png');
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− | display: flex;
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− | flex-direction: column;
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− | justify-content: flex-end;
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− | z-index: 0;
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− | #home>.pageHead::before {
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− | background-color: rgba(0, 36, 78, .5);
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− | #home>.pageHead>.pageHead-content {
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− | text-align: center;
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− | display: flex;
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− | flex-direction: column;
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− | #home>.pageHead>.pageHead-content>.pageHead-content__title {
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− | font-size: 5rem;
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− | color: #ffffff;
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− | #home>.pageHead>.pageHead-content>.pageHead-content__description {
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− | margin: 6rem 10% 4rem 10%;
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− | font-size: 1rem;
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− | color: #ffffff;
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− | #home>.pageContent {
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− | display: flex;
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− | flex-direction: row;
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− | min-height: 60vh;
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− | #home>.pageContent>.pageContent-side {
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− | width: 20%;
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− | max-width: 200px;
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− |
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− | #navlist {
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− | background-color: var(--dark);
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− | padding: 2rem .5rem 4rem .5rem;
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− | min-height: 55vh;
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− | border-bottom-right-radius: 2rem;
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− | position: relative;
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− | text-align: center;
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− | display: flex;
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− | flex-direction: column;
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− | #navlist>.pageContent-navlist__head {
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− | padding: 1rem 0 1rem 0;
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− | #navlist>.pageContent-navlist__head>.logo {
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− | margin: 1rem auto;
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− | width: 3.5rem;
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− | height: 3.5rem;
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− | border-radius: 50%;
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− | background-color: #dfdfdf;
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− | #navlist>.pageContent-navlist__head>.title {
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− | font-weight: bold;
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− | #navlist>.pageContent-navlist__item {
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− | #navlist>.pageContent-navlist__item:hover,
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− | #navlist>.active {
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− | #pagecontent {
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− | #pagecontent .pageContent-main__title {
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− | #pagecontent .pageContent-main__subtitle {
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− | font-size: 1.5rem;
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− | #pagecontent .pageContent-main__title::after {
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− | #pagecontent .pageContent-main__textBox {
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− | #pagecontent .pageContent-main__textBox .name {
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− | #pagecontent .pageContent-main__textBox .image {
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− | margin: 2rem 10%;
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− | .pageContent-side .mytoc {
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− | position: -webkit-sticky;
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− | position: sticky;
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− | top: 12rem;
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− | background: transparent;
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− | padding: 1.5rem;
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− | border: none;
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− |
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− | .pageContent-side .mytoc .toc_list {
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− | font-size: .8rem;
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− | .pageContent-side .mytoc .toc_list .toclist__item a {
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− | color: #888;
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− | text-decoration: none;
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− | .pageContent-side .mytoc .toc_list .toclist__item a:hover {
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− | color: var(--primary)
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− | }
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− |
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− | @media screen and (max-width: 800px) {
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− | #home>.screen>#group1>.title {
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− | width: 100%;
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− | }
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− | #navbar>.logo {
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− | padding: 0.5rem 0;
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− | }
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− | #collapse {
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− | height: 0;
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− | }
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− | #home>.pageContent>.pageContent-side {
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− | display: none;
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− | }
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− | #home>.screen>#group1 {
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− | padding-top: 5vh;
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− | }
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− | #home>.pageContent>.pageContent-main>.profilelist>.profile {
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− | width: calc(100% / 3)
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− | }
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− | }
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− | </style>
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− | <h2 class="pageContent-main__title">
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− | <!--put tile here, <h2>title</h2>, class="pageContent-main__title" means it is the main title-->
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| LOV | | LOV |
| </h2> | | </h2> |
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| <div class="pageContent-main__textBox"> | | <div class="pageContent-main__textBox"> |
| <!--all the content must included in this div--> | | <!--all the content must included in this div--> |
− | <img class="image" src="https://2021.igem.org/wiki/images/f/f3/T--CSU_CHINA--tupian8.png"> | + | <p style="text-align: center;"> |
| + | <img width="400px" src="https://2021.igem.org/wiki/images/f/f3/T--CSU_CHINA--tupian8.png"></p> |
| <!--put image's url here--> | | <!--put image's url here--> |
| <p style="width: 80%;text-align:center;font-size: .9rem; margin: -1rem auto 1rem auto; color: #888;">Fig.1 The model diagram of LOV-VP16</p> | | <p style="width: 80%;text-align:center;font-size: .9rem; margin: -1rem auto 1rem auto; color: #888;">Fig.1 The model diagram of LOV-VP16</p> |
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| </p> | | </p> |
| </div> | | </div> |
− | <body> | + | </body> |
− | <html> | + | </html> |
| + | |
| <!-- Add more about the biology of this part here | | <!-- Add more about the biology of this part here |
| ===Usage and Biology=== | | ===Usage and Biology=== |
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| <span class='h3bb'>Sequence and Features</span> | | <span class='h3bb'>Sequence and Features</span> |
| <partinfo>BBa_K3734006 SequenceAndFeatures</partinfo> | | <partinfo>BBa_K3734006 SequenceAndFeatures</partinfo> |
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| <!-- Uncomment this to enable Functional Parameter display | | <!-- Uncomment this to enable Functional Parameter display |
LOV is a kind of light-activated protein. If you shine blue light on it, it will change its own structure and will be able to combined with GI.
LOV is a photosensitive protein, the structure will alter under 450nm blue light exposure and form a dimer with GI. We used LOV as a part of our blue light switch.
The duration of the combination of GI and LOV after the blue light exposure will alter as the illuminate method and the intensity of the blue light differ. We used strong light for a 30min exposure and then use weak light for another 30min
exposure.
[1]Masayuki Yazawa , Amir M Sadaghiani, Brian Hsueh.Induction of protein-protein interactions in live cells using light[J].Nat Biotechnol. 2009 Oct;27(10):941-5.