Difference between revisions of "Part:BBa K3882003"
 
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Part 2 provides the function to up-regulate the expression of  pvdQ to convert N-Acyl Homoserine Lactone (AHL) molecule into L-Homoserine Lactone (LHL) which is incapable of generating QS signals. T7 promoter serves as starting the whole synthetic gene. Lac operator is used for releasing LuxR. PVDQ protein functions as restraining P. aeruginosa from growing and activating GFP gene. GFP is a green fluorescence reporting gene.
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Part 2 provides the function to up-regulate the expression of  pvdQ to convert N-Acyl Homoserine Lactone (AHL) molecule into L-Homoserine Lactone (LHL) which is incapable of generating QS signals. T7 promoter serves as starting the whole synthetic gene. Lac operator is used for releasing LuxR. PVDQ protein functions as restraining <i>P. aeruginosa</i>  from growing and activating GFP gene. GFP is a green fluorescence reporting gene.
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===Result===
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The results from the plates are obvious. By counting the numbers of bacterial colonies, we found that initially LL-37 peptide is able to sterilize part of the <i>P. aeruginosa</i> , but the combined use of PVDQ from <i> E. bsuahlterminator</i> and LL-37 peptide can sterilize <i>P. aeruginosa</i> more efficiently and validly.
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<br/><li style="display: inline-block;"> [[File:Compositeexperiment.png|thumb|center|800px|'''Fig.1. 3 groups in the experiment and each group contains 3 individuals. (The last individual of the last group that is with LL-37 + PVDQ was not evenly distributed, but the result can still be obvious).]]
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<br/><li style="display: inline-block;"> [[File:Numbersofbacterial .png|thumb|center|800px|'''Fig.2 Numbers of bacterial colonies on average of plates of each group.]]
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Latest revision as of 08:36, 20 October 2021


E. bsuahlterminator (Part2)

Part 2 provides the function to up-regulate the expression of pvdQ to convert N-Acyl Homoserine Lactone (AHL) molecule into L-Homoserine Lactone (LHL) which is incapable of generating QS signals. T7 promoter serves as starting the whole synthetic gene. Lac operator is used for releasing LuxR. PVDQ protein functions as restraining P. aeruginosa from growing and activating GFP gene. GFP is a green fluorescence reporting gene.

Result

The results from the plates are obvious. By counting the numbers of bacterial colonies, we found that initially LL-37 peptide is able to sterilize part of the P. aeruginosa , but the combined use of PVDQ from E. bsuahlterminator and LL-37 peptide can sterilize P. aeruginosa more efficiently and validly.


  • Fig.1. 3 groups in the experiment and each group contains 3 individuals. (The last individual of the last group that is with LL-37 + PVDQ was not evenly distributed, but the result can still be obvious).

  • Fig.2 Numbers of bacterial colonies on average of plates of each group.


    Sequence and Features


    Assembly Compatibility:
    • 10
      COMPATIBLE WITH RFC[10]
    • 12
      COMPATIBLE WITH RFC[12]
    • 21
      COMPATIBLE WITH RFC[21]
    • 23
      COMPATIBLE WITH RFC[23]
    • 25
      COMPATIBLE WITH RFC[25]
    • 1000
      COMPATIBLE WITH RFC[1000]