Difference between revisions of "Part:BBa K3734006"
| Line 11: | Line 11: | ||
<body> | <body> | ||
| − | + | <h2 class="pageContent-main__title"><!--put tile here, <h2>title</h2>, class="pageContent-main__title" means it is the main title--> | |
| − | + | ||
LOV | LOV | ||
</h2> | </h2> | ||
| Line 26: | Line 25: | ||
<div class="pageContent-main__textBox"> | <div class="pageContent-main__textBox"> | ||
<!--all the content must included in this div--> | <!--all the content must included in this div--> | ||
| − | <img width="400px" src="https://2021.igem.org/wiki/images/f/f3/T--CSU_CHINA--tupian8.png"> | + | <p style="text-align: center;"> |
| + | <img width="400px" src="https://2021.igem.org/wiki/images/f/f3/T--CSU_CHINA--tupian8.png"></p> | ||
<!--put image's url here--> | <!--put image's url here--> | ||
<p style="width: 80%;text-align:center;font-size: .9rem; margin: -1rem auto 1rem auto; color: #888;">Fig.1 The model diagram of LOV-VP16</p> | <p style="width: 80%;text-align:center;font-size: .9rem; margin: -1rem auto 1rem auto; color: #888;">Fig.1 The model diagram of LOV-VP16</p> | ||
| Line 49: | Line 49: | ||
</p> | </p> | ||
</div> | </div> | ||
| − | |||
<body> | <body> | ||
<html> | <html> | ||
| Line 59: | Line 58: | ||
<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
<partinfo>BBa_K3734006 SequenceAndFeatures</partinfo> | <partinfo>BBa_K3734006 SequenceAndFeatures</partinfo> | ||
| − | |||
<!-- Uncomment this to enable Functional Parameter display | <!-- Uncomment this to enable Functional Parameter display | ||
Revision as of 08:35, 20 October 2021
LOV
LOV is a kind of light-activated protein. If you shine blue light on it, it will change its own structure and will be able to combined with GI.
LOV
LOV is a photosensitive protein, the structure will alter under 450nm blue light exposure and form a dimer with GI. We used LOV as a part of our blue light switch.
1.Pattern diagram
Fig.1 The model diagram of LOV-VP16
2.Caution
The duration of the combination of GI and LOV after the blue light exposure will alter as the illuminate method and the intensity of the blue light differ. We used strong light for a 30min exposure and then use weak light for another 30min exposure.
Reference：
[1]Masayuki Yazawa , Amir M Sadaghiani, Brian Hsueh.Induction of protein-protein interactions in live cells using light[J].Nat Biotechnol. 2009 Oct;27(10):941-5.