Difference between revisions of "Part:BBa K3735004"
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<partinfo>BBa_K3735004 short</partinfo> | <partinfo>BBa_K3735004 short</partinfo> | ||
− | In order to find an appropriate expression intensity to achieve balance between metabolic burden and yields of products, NAU-CHINA designed three composite parts including three promoters with different intensity(twins). This composite part contains the promoter with middle intensity ( | + | In order to find an appropriate expression intensity to achieve balance between metabolic burden and yields of products, NAU-CHINA designed three composite parts including three promoters with different intensity(twins). This composite part contains the promoter with middle intensity (BBa_J23101). With promoter and RBS, the composite part can be directly imported into plasmid and express <i>SefA</i> gene. If you want to learn about the detailed introduction of <i>SefA</i>, you can click the link below.(https://parts.igem.org/Part:BBa_K3735001) |
+ | |||
+ | https://2021.igem.org/wiki/images/2/2a/T--NAU-CHINA--ES.fig.4.png | ||
+ | <br> | ||
+ | Figure.1 growth curve of different promoters in different selenite concentrations | ||
+ | |||
+ | |||
+ | https://2021.igem.org/wiki/images/2/24/T--NAU-CHINA--ES.fig.5.png | ||
+ | <br> | ||
+ | Figure.2 SeNPs output of different promoters in different selenite concentrations | ||
Latest revision as of 07:34, 20 October 2021
Coding sequence
In order to find an appropriate expression intensity to achieve balance between metabolic burden and yields of products, NAU-CHINA designed three composite parts including three promoters with different intensity(twins). This composite part contains the promoter with middle intensity (BBa_J23101). With promoter and RBS, the composite part can be directly imported into plasmid and express SefA gene. If you want to learn about the detailed introduction of SefA, you can click the link below.(https://parts.igem.org/Part:BBa_K3735001)
Figure.1 growth curve of different promoters in different selenite concentrations
Figure.2 SeNPs output of different promoters in different selenite concentrations
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1537
Illegal BglII site found at 2854 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 329
Illegal NgoMIV site found at 644
Illegal NgoMIV site found at 770
Illegal NgoMIV site found at 1151
Illegal NgoMIV site found at 1574
Illegal NgoMIV site found at 2675
Illegal NgoMIV site found at 2924
Illegal AgeI site found at 671
Illegal AgeI site found at 1217
Illegal AgeI site found at 1373
Illegal AgeI site found at 1514
Illegal AgeI site found at 1937
Illegal AgeI site found at 2294
Illegal AgeI site found at 2465 - 1000COMPATIBLE WITH RFC[1000]