Difference between revisions of "Part:BBa K3889026"

 
 
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<partinfo>BBa_K3889026 short</partinfo>
 
<partinfo>BBa_K3889026 short</partinfo>
  
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ytvA protein is light-sensitive thereby helping have a light-induced protein expression system.
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ytvA protein is a blue light sensor thereby helps in light-induced protein expression.
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<!-- Add more about the biology of this part here
 
 
===Usage and Biology===
 
===Usage and Biology===
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Ytva is continuously produced in the dark state and stays in the dark state until it comes in contact with blue light. It has two domains namely LOV (light oxygen voltage) domain and a C-terminal STAS (sulphate transporter and anti-sigma factor antagonist) domain. LOV domain from these binds to FMN (Flavin Mononucleotide) forming a molecule that has maximum absorbance of 448nm in the dark. Hence, the illumination of blue light triggers a photocycle, which includes the formation of a signalling state that converts back to a dark state with a recovery time of 2600 seconds. Ytva-LOV is considered a monomer and is found to exist as a homodimer.[2]
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In activated state, YtvA is able to free SigB from RsbW through a cascade of reactions between Regulatory SigB (Rsb) factors  as shown:[1]
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[[File:T--IISER-Tirupati India--sigB activation.png]]
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Through this process, promoters dependent on sigB like <partinfo>BBa_K3889013</partinfo> starts expressing genes. Overexpression of YtvA can be used to produce a system sensitive to blue light.
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This is a modified version of <partinfo>BBa_K143037</partinfo> specific to our project's assembly. Mutated Bsa1 and HindIII Sites to make an assembly compatible part.
  
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K3889026 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K3889026 SequenceAndFeatures</partinfo>
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<partinfo>BBa_K3889026 parameters</partinfo>
 
<partinfo>BBa_K3889026 parameters</partinfo>
 
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===References===
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1. van der Steen, J. B., Nakasone, Y., Hendriks, J., & Hellingwerf, K. J. (2013). Modeling the functioning of YtvA in the general stress response in Bacillus subtilis. In Molecular BioSystems (Vol. 9, Issue 9, p. 2331). Royal Society of Chemistry (RSC). https://doi.org/10.1039/c3mb70124g
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2. Möglich, A., & Moffat, K. (2007). Structural basis for light-dependent signaling in the dimeric LOV domain of the photosensor YtvA. Journal of molecular biology, 373(1), 112–126. https://doi.org/10.1016/j.jmb.2007.07.039

Latest revision as of 07:27, 20 October 2021


Bacillus subtilis ytvA protein

ytvA protein is a blue light sensor thereby helps in light-induced protein expression.


Usage and Biology

Ytva is continuously produced in the dark state and stays in the dark state until it comes in contact with blue light. It has two domains namely LOV (light oxygen voltage) domain and a C-terminal STAS (sulphate transporter and anti-sigma factor antagonist) domain. LOV domain from these binds to FMN (Flavin Mononucleotide) forming a molecule that has maximum absorbance of 448nm in the dark. Hence, the illumination of blue light triggers a photocycle, which includes the formation of a signalling state that converts back to a dark state with a recovery time of 2600 seconds. Ytva-LOV is considered a monomer and is found to exist as a homodimer.[2]

In activated state, YtvA is able to free SigB from RsbW through a cascade of reactions between Regulatory SigB (Rsb) factors as shown:[1] T--IISER-Tirupati India--sigB activation.png

Through this process, promoters dependent on sigB like BBa_K3889013 starts expressing genes. Overexpression of YtvA can be used to produce a system sensitive to blue light.

This is a modified version of BBa_K143037 specific to our project's assembly. Mutated Bsa1 and HindIII Sites to make an assembly compatible part.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]



References

1. van der Steen, J. B., Nakasone, Y., Hendriks, J., & Hellingwerf, K. J. (2013). Modeling the functioning of YtvA in the general stress response in Bacillus subtilis. In Molecular BioSystems (Vol. 9, Issue 9, p. 2331). Royal Society of Chemistry (RSC). https://doi.org/10.1039/c3mb70124g

2. Möglich, A., & Moffat, K. (2007). Structural basis for light-dependent signaling in the dimeric LOV domain of the photosensor YtvA. Journal of molecular biology, 373(1), 112–126. https://doi.org/10.1016/j.jmb.2007.07.039