Difference between revisions of "Part:BBa K3889028"
 
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<partinfo>BBa_K3889028 short</partinfo>
 
<partinfo>BBa_K3889028 short</partinfo>
  
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Tag specific protease that can be used for regulating recombinant protein expression.
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mfLon is a Lon protease present in most bacteria from <i>Mesoplasma florum</i>. It can function independently of the host, providing functionality in a variety of bacteria. It can degrade proteins at different rates depending on the protein degradation tag attached to it [1].
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<groupparts>iGEM016 Dundee_Schools<groupparts>
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<groupparts>iGEM21 iGEM21_IISER-Tirupati_India [Favorites] </groupparts>
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This is a modified version of <partinfo>BBa_K3889020</partinfo> specific to our project's assembly. Mutated multiple RE sites to make it assembly compatible.
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===Usage and Biology===
 
===Usage and Biology===
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<partinfo>BBa_K3889028 parameters</partinfo>
 
<partinfo>BBa_K3889028 parameters</partinfo>
 
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===References===
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1. Cameron, D. E., & Collins, J. J. (2014). Tunable protein degradation in bacteria. Nature biotechnology, 32(12), 1276–1281. https://doi.org/10.1038/nbt.3053

Latest revision as of 07:26, 20 October 2021


Modified mfLon protease

mfLon is a Lon protease present in most bacteria from Mesoplasma florum. It can function independently of the host, providing functionality in a variety of bacteria. It can degrade proteins at different rates depending on the protein degradation tag attached to it [1].


This is a modified version of BBa_K3889020 specific to our project's assembly. Mutated multiple RE sites to make it assembly compatible.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


References

1. Cameron, D. E., & Collins, J. J. (2014). Tunable protein degradation in bacteria. Nature biotechnology, 32(12), 1276–1281. https://doi.org/10.1038/nbt.3053