Difference between revisions of "Part:BBa K3507003"
 
(2 intermediate revisions by one other user not shown)
Line 3: Line 3:
 
<partinfo>BBa_K3507003 short</partinfo>
 
<partinfo>BBa_K3507003 short</partinfo>
  
−
YqcF is  a small protein acting as an antitoxin in a type II toxin-antitoxin (TA) system described in Bacillus subtilis. This protein is capable of binding to its counterpart YpcG, thereby preventing its DNase activity in the cell. Most likely, this interaction occurs through binding to the LXG domain of YpcG (Holberger et al., 2012). However, it has been shown that it doesn’t exhibit antitoxic effects on other toxins that contain the same LXG domain (REF).  
+
YqcF is  a small protein acting as an antitoxin in a type II toxin-antitoxin (TA) system described in Bacillus subtilis. This protein is capable of binding to its counterpart YqcG, thereby preventing its DNase activity in the cell. Most likely, this interaction occurs through binding to the LXG domain of YpcG (Holberger et al., 2012). However, it has been shown that it doesn’t exhibit antitoxic effects on other toxins that contain the same LXG domain (Holberger et al., 2012).  
 
For our iGEM project we intend on expressing this antitoxin under the control of a solanine dependent promoter with the purpose of rescuing cells that are located in the proximity of the potato root exudate. Solanine is a main and specific component of potato root exudates and, by putting the production of YpcF under the control of solanine dependent promoter, we can only allow its production in the potato environment. The cells will constitutively express the toxin part of the system (YqcG) but their survival will be dependent on the presence of solanine. By this, we hope to implement a kill switch system that allows the B. mycoides to survive only in the designated area.  
 
For our iGEM project we intend on expressing this antitoxin under the control of a solanine dependent promoter with the purpose of rescuing cells that are located in the proximity of the potato root exudate. Solanine is a main and specific component of potato root exudates and, by putting the production of YpcF under the control of solanine dependent promoter, we can only allow its production in the potato environment. The cells will constitutively express the toxin part of the system (YqcG) but their survival will be dependent on the presence of solanine. By this, we hope to implement a kill switch system that allows the B. mycoides to survive only in the designated area.  
  
Line 25: Line 25:
 
<!-- -->
 
<!-- -->
  
−
[[File:T--Groningen--yqcgf.png|500px|thumb|centre|Figure1. The interaction of the two components of the YqcGF system. (Adapted from Brantl et al., 2019)]]
+
[[File:T--Groningen--yqcgf.png|500px|thumb|centre|Figure 1. The interaction of the two components of the YqcGF system. (Adapted from Brantl et al., 2019)]]
  
 
<h5>Bibliography</h5>
 
<h5>Bibliography</h5>
 +
<p>1.Holberger LE, Garza-sánchez F, Lamoureux J, Low DA, Hayes CS. A novel family of toxin / antitoxin proteins in Bacillus species. FEBS Lett [Internet]. 2012;586(2):132–6. Available from: http://dx.doi.org/10.1016/j.febslet.2011.12.020</p>
 +
<p>2.Brantl S, Müller P. Toxin-antitoxin systems in bacillus subtilis. Toxins (Basel). 2019;11(5).</p>
 +
 +
 +
==Modifications in Sequence : iGEM21_IISER-Tirupati_India==
 +
 +
<partinfo>BBa_K3889093</partinfo> is a modified version specific to our project's assembly. Mutated BgIII site to make assembly compatible part.

Latest revision as of 06:49, 20 October 2021


Antitoxin part of a toxin-antitoxin system of B. subtilis

YqcF is a small protein acting as an antitoxin in a type II toxin-antitoxin (TA) system described in Bacillus subtilis. This protein is capable of binding to its counterpart YqcG, thereby preventing its DNase activity in the cell. Most likely, this interaction occurs through binding to the LXG domain of YpcG (Holberger et al., 2012). However, it has been shown that it doesn’t exhibit antitoxic effects on other toxins that contain the same LXG domain (Holberger et al., 2012). For our iGEM project we intend on expressing this antitoxin under the control of a solanine dependent promoter with the purpose of rescuing cells that are located in the proximity of the potato root exudate. Solanine is a main and specific component of potato root exudates and, by putting the production of YpcF under the control of solanine dependent promoter, we can only allow its production in the potato environment. The cells will constitutively express the toxin part of the system (YqcG) but their survival will be dependent on the presence of solanine. By this, we hope to implement a kill switch system that allows the B. mycoides to survive only in the designated area.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 519
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]



Figure 1. The interaction of the two components of the YqcGF system. (Adapted from Brantl et al., 2019)
Bibliography

1.Holberger LE, Garza-sánchez F, Lamoureux J, Low DA, Hayes CS. A novel family of toxin / antitoxin proteins in Bacillus species. FEBS Lett [Internet]. 2012;586(2):132–6. Available from: http://dx.doi.org/10.1016/j.febslet.2011.12.020

2.Brantl S, Müller P. Toxin-antitoxin systems in bacillus subtilis. Toxins (Basel). 2019;11(5).


Modifications in Sequence : iGEM21_IISER-Tirupati_India

BBa_K3889093 is a modified version specific to our project's assembly. Mutated BgIII site to make assembly compatible part.