Difference between revisions of "Part:BBa K3992002"

Latest revision as of 04:14, 20 October 2021

GS linker

Profile

Name: GS linker

Base Pairs: 57bp

Origin: Synthetic

Properties: A linker to linked different proteins.

Usage and Biology

BBa_K3992002 is a part of GS linker. It can link different proteins to make them become a fusion protein. In our group, we use this part to link VP7 protein and LTB protein.

Construct Design

VP7-LTB is linked by GS linker (Figure 1).

Figure 1 The vp7-GS linker-LTB box.

Experimental approach

Polymerase Chain Reaction

A PCR verification was performed to confirm whether the sequence of synthesized VP7-LTB is cerrect. Agarose gel electrophoresis was used to assess the PCR’s result. According to the 15000 bp DNA marker, the PCR amplified DNA fragments possess the desired right size.

Figure 2 PCR verification of VP7 and VP7-LTB.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 5: / Line 5: @@
 GS linker
-<!-- Add more about the biology of this part here
+=== Profile ===
+====Name: GS linker====
+====Base Pairs: 57bp====
+====Origin: Synthetic====
+====Properties: A linker to linked different proteins.====
 ===Usage and Biology===
+BBa_K3992002 is a part of GS linker. It can link different proteins to make them become a fusion protein. In our group, we use this part to link VP7 protein and LTB protein.
+===Construct Design===
+VP7-LTB is linked by GS linker (Figure 1).
+[[File:T--Shanghai high school--BBa K3992002-Figure1.png|500px|thumb|center|Figure 1 The vp7-GS linker-LTB box.]]
+=== Experimental approach ===
+====Polymerase Chain Reaction====
+A PCR verification was performed to confirm whether the sequence of synthesized VP7-LTB is cerrect. Agarose gel electrophoresis was used to assess the PCR’s result. According to the 15000 bp DNA marker, the PCR amplified DNA fragments possess the desired right size.
+[[File:T--Shanghai high school--BBa K3992002-Figure2.png|500px|thumb|center|Figure 2 PCR verification of VP7 and VP7-LTB.]]
 <!-- -->