Difference between revisions of "Part:BBa K3745000:Design"

(→‎Design Notes)
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===Design Notes===
 
===Design Notes===
 
This composite part is used to select desired mutants. The more the presence of rhamnose, the more the production of ampicillin, which can assist the cell to survive better.
 
This composite part is used to select desired mutants. The more the presence of rhamnose, the more the production of ampicillin, which can assist the cell to survive better.
 +
 +
This part is originally carried on pSB1C3 backbone.
  
 
===Source===
 
===Source===
  
−
Made up of basic parts
+
Improved from BBa_K914003
  
 
===References===
 
===References===
 +
1. Kelly et al, 2016 Synthetic Chemical Inducers and Genetic Decoupling Enable Orthogonal Control of the rhaBAD Promoter
 +
2. Kelly et al, 2018 A Rhamnose-Inducible System for Precise and Temporal Control of Gene Expression in Cyanobacteria
 +
3. Hjelm, 2017 Tailoring Escherichia coli for the L‑Rhamnose PBAD Promoter-Based Production of Membrane and Secretory Proteins

Revision as of 02:16, 20 October 2021


Prha-Amp


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 869


Design Notes

This composite part is used to select desired mutants. The more the presence of rhamnose, the more the production of ampicillin, which can assist the cell to survive better.

This part is originally carried on pSB1C3 backbone.

Source

Improved from BBa_K914003

References

1. Kelly et al, 2016 Synthetic Chemical Inducers and Genetic Decoupling Enable Orthogonal Control of the rhaBAD Promoter 2. Kelly et al, 2018 A Rhamnose-Inducible System for Precise and Temporal Control of Gene Expression in Cyanobacteria 3. Hjelm, 2017 Tailoring Escherichia coli for the L‑Rhamnose PBAD Promoter-Based Production of Membrane and Secretory Proteins