Difference between revisions of "Part:BBa K3726056:Design"

Revision as of 01:16, 20 October 2021

Lv.1_PKMCG-B

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal EcoRI site found at 2173
Illegal EcoRI site found at 2614
Illegal EcoRI site found at 4373
Illegal EcoRI site found at 5489
Illegal EcoRI site found at 5573
Illegal EcoRI site found at 8628
Illegal PstI site found at 2758
Illegal PstI site found at 2857
Illegal PstI site found at 3442
Illegal PstI site found at 4400
Illegal PstI site found at 4628
Illegal PstI site found at 6138
Illegal PstI site found at 7152
12
INCOMPATIBLE WITH RFC[12]
Illegal EcoRI site found at 2173
Illegal EcoRI site found at 2614
Illegal EcoRI site found at 4373
Illegal EcoRI site found at 5489
Illegal EcoRI site found at 5573
Illegal EcoRI site found at 8628
Illegal NheI site found at 5712
Illegal PstI site found at 2758
Illegal PstI site found at 2857
Illegal PstI site found at 3442
Illegal PstI site found at 4400
Illegal PstI site found at 4628
Illegal PstI site found at 6138
Illegal PstI site found at 7152
21
INCOMPATIBLE WITH RFC[21]
Illegal EcoRI site found at 2173
Illegal EcoRI site found at 2614
Illegal EcoRI site found at 4373
Illegal EcoRI site found at 5489
Illegal EcoRI site found at 5573
Illegal EcoRI site found at 8628
Illegal BglII site found at 1115
Illegal BglII site found at 3155
Illegal BglII site found at 4519
Illegal BamHI site found at 3253
Illegal BamHI site found at 3268
Illegal BamHI site found at 6967
Illegal BamHI site found at 7425
Illegal BamHI site found at 8398
Illegal XhoI site found at 337
Illegal XhoI site found at 1945
Illegal XhoI site found at 2590
Illegal XhoI site found at 3064
Illegal XhoI site found at 5594
Illegal XhoI site found at 6261
Illegal XhoI site found at 6394
23
INCOMPATIBLE WITH RFC[23]
Illegal EcoRI site found at 2173
Illegal EcoRI site found at 2614
Illegal EcoRI site found at 4373
Illegal EcoRI site found at 5489
Illegal EcoRI site found at 5573
Illegal EcoRI site found at 8628
Illegal PstI site found at 2758
Illegal PstI site found at 2857
Illegal PstI site found at 3442
Illegal PstI site found at 4400
Illegal PstI site found at 4628
Illegal PstI site found at 6138
Illegal PstI site found at 7152
25
INCOMPATIBLE WITH RFC[25]
Illegal EcoRI site found at 2173
Illegal EcoRI site found at 2614
Illegal EcoRI site found at 4373
Illegal EcoRI site found at 5489
Illegal EcoRI site found at 5573
Illegal EcoRI site found at 8628
Illegal PstI site found at 2758
Illegal PstI site found at 2857
Illegal PstI site found at 3442
Illegal PstI site found at 4400
Illegal PstI site found at 4628
Illegal PstI site found at 6138
Illegal PstI site found at 7152
Illegal NgoMIV site found at 2701
Illegal NgoMIV site found at 4090
Illegal NgoMIV site found at 4264
Illegal NgoMIV site found at 4414
Illegal NgoMIV site found at 7588
Illegal AgeI site found at 4715
1000
COMPATIBLE WITH RFC[1000]

Design Notes

This is a composite part that corresponds with a level 1 MoClo part in accordance with the marburg collection standard. It is a transcriptional unit for the expression of the part “BBa_K3726038” L0_MCGII which contains the other half part of the MCG cycle.

The other half of the MCG cycle is going to be expressed in the part PKMCGII_Lv1 which has two variants “BBa_K3726053” L1_PKMCGII-A and “BBa_K3726054” L1_PKMCGII-B. The Malate-acetyl-CoA-Glycerate (MCG) cycle aims to the production of acetyl-CoA with high efficiency coupled to carbon capture from bicarbonate anion and with recovery of metabolic intermediates produced during photorespiration. (H. Yu, X. Li, F. Duchoud, D. Chuang and J. Liao, "Augmenting the Calvin–Benson–Bassham cycle by a synthetic malyl-CoA-glycerate carbon fixation pathway", 2021).

The part is flanked by two homology regions “ BBa_K3726110” 5CON1(H)_nNS4-up (PCC 11801) and “BBa_K3726111” 3CON5(H)_nNS4-down (PCC 11801) for the double homologous recombination within the genome of PCC 11801.

Likewise, an ampicillin resistance cassette is included downstream the transcriptional unit region.

Expression is controlled by the strong constitutive promoter for cyanobacteria, PS-PR “BBa_K3726084” and the strong synthetic RBS, RBS_Ppc “BBa_K3726091”.

Source

This construct has been made by golden gate reaction.

References

H. Yu, X. Li, F. Duchoud, D. Chuang and J. Liao, "Augmenting the Calvin–Benson–Bassham cycle by a synthetic malyl-CoA-glycerate carbon fixation pathway", 2021

@@ Line 7: / Line 7: @@
 ===Design Notes===
-.
+This is a composite part that corresponds with a level 1 MoClo part in accordance with  the marburg collection standard. It is a transcriptional unit for the expression of the part “BBa_K3726038” L0_MCGII which contains the other half part of the MCG cycle.
+The other half of the MCG cycle is going to be expressed in the part PKMCGII_Lv1 which has two variants “BBa_K3726053” L1_PKMCGII-A and “BBa_K3726054” L1_PKMCGII-B. The Malate-acetyl-CoA-Glycerate (MCG) cycle aims to the production of acetyl-CoA with high efficiency coupled to carbon capture from bicarbonate anion and with recovery of metabolic intermediates produced during photorespiration. (H. Yu, X. Li, F. Duchoud, D. Chuang and J. Liao, "Augmenting the Calvin–Benson–Bassham cycle by a synthetic malyl-CoA-glycerate carbon fixation pathway", 2021).
+The part is flanked by two homology regions “    BBa_K3726110” 5CON1(H)_nNS4-up (PCC 11801) and “BBa_K3726111” 3CON5(H)_nNS4-down (PCC 11801) for the double homologous recombination within the genome of PCC 11801.
+Likewise, an ampicillin resistance cassette is included downstream the transcriptional unit region.
+Expression is controlled by the strong constitutive promoter for cyanobacteria, PS-PR “BBa_K3726084”  and the strong synthetic RBS, RBS_Ppc  “BBa_K3726091”.
 ===Source===
-.
+This construct has been made by golden gate reaction.
 ===References===
+H. Yu, X. Li, F. Duchoud, D. Chuang and J. Liao, "Augmenting the Calvin–Benson–Bassham cycle by a synthetic malyl-CoA-glycerate carbon fixation pathway", 2021