Difference between revisions of "Part:BBa K3726054:Design"

 
 
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===Design Notes===
 
===Design Notes===
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The part is a MoClo Lv.1 part, assembled in in the acceptor Lv1/2 entry vector whose ori is “BBa_K2560036”, and it's resistance cassette is “BBa_K3726039” (MARK_Lv0_AmpR).  This part has been assembled following the marburg collection standard, then the transcriptional unit is flanked by the parts “BBa_K3726112” 5CON1(H)_nNS5-up (PCC 11801) and “BBa_K3726113” 3CON5(H)_nNS5-down (PCC 11801) allowing the Integration within the PCC 11801 Genome.
  
 
===Source===
 
===Source===
  
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.
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This construct has been made by golden gate reaction.  
  
 
===References===
 
===References===
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H. Yu, X. Li, F. Duchoud, D. Chuang and J. Liao, "Augmenting the Calvin–Benson–Bassham cycle by a synthetic malyl-CoA-glycerate carbon fixation pathway", 2021.

Latest revision as of 01:02, 20 October 2021


Lv.1_PKMCG-B


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 9319
    Illegal SpeI site found at 10282
    Illegal PstI site found at 575
    Illegal PstI site found at 839
    Illegal PstI site found at 4205
    Illegal PstI site found at 4834
    Illegal PstI site found at 5331
    Illegal PstI site found at 5930
    Illegal PstI site found at 6868
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 9319
    Illegal NheI site found at 8971
    Illegal NheI site found at 8998
    Illegal SpeI site found at 10282
    Illegal PstI site found at 575
    Illegal PstI site found at 839
    Illegal PstI site found at 4205
    Illegal PstI site found at 4834
    Illegal PstI site found at 5331
    Illegal PstI site found at 5930
    Illegal PstI site found at 6868
    Illegal NotI site found at 9325
    Illegal NotI site found at 10289
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 9319
    Illegal BamHI site found at 505
    Illegal BamHI site found at 2744
    Illegal BamHI site found at 9864
    Illegal XhoI site found at 9195
    Illegal XhoI site found at 11313
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 9319
    Illegal suffix found in sequence at 10282
    Illegal PstI site found at 575
    Illegal PstI site found at 839
    Illegal PstI site found at 4205
    Illegal PstI site found at 4834
    Illegal PstI site found at 5331
    Illegal PstI site found at 5930
    Illegal PstI site found at 6868
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 9319
    Illegal XbaI site found at 9334
    Illegal SpeI site found at 10282
    Illegal PstI site found at 575
    Illegal PstI site found at 839
    Illegal PstI site found at 4205
    Illegal PstI site found at 4834
    Illegal PstI site found at 5331
    Illegal PstI site found at 5930
    Illegal PstI site found at 6868
    Illegal NgoMIV site found at 2321
    Illegal NgoMIV site found at 4430
    Illegal NgoMIV site found at 4890
    Illegal NgoMIV site found at 8786
    Illegal NgoMIV site found at 8928
    Illegal AgeI site found at 6443
    Illegal AgeI site found at 6526
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 9349
    Illegal BsaI.rc site found at 10266


Design Notes

The part is a MoClo Lv.1 part, assembled in in the acceptor Lv1/2 entry vector whose ori is “BBa_K2560036”, and it's resistance cassette is “BBa_K3726039” (MARK_Lv0_AmpR). This part has been assembled following the marburg collection standard, then the transcriptional unit is flanked by the parts “BBa_K3726112” 5CON1(H)_nNS5-up (PCC 11801) and “BBa_K3726113” 3CON5(H)_nNS5-down (PCC 11801) allowing the Integration within the PCC 11801 Genome.

Source

This construct has been made by golden gate reaction.

References

H. Yu, X. Li, F. Duchoud, D. Chuang and J. Liao, "Augmenting the Calvin–Benson–Bassham cycle by a synthetic malyl-CoA-glycerate carbon fixation pathway", 2021.