Difference between revisions of "Part:BBa K3726053"

 
 
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<partinfo>BBa_K3726053 short</partinfo>
 
<partinfo>BBa_K3726053 short</partinfo>
  
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This is a composite part that corresponds with a level 1 MoClo part in accordance with  the marburg collection standard. It is a transcriptional unit for the expression of the part “BBa_K3726028” L0_PKMCG  which contains the half part of the MCG cycle and the enzymes that belong to the phosphoketolase pathway (X. Liu, R. Miao, P. Lindberg and P. Lindblad, "Modular engineering for efficient photosynthetic biosynthesis of 1-butanol from CO2in cyanobacteria", 2021).
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The other half of the MCG cycle is going to be expressed in the part MCGII_Lv1 which has two variants “BBa_K3726055” L1_MCGII-A and “BBa_K3726056” L1_MCGII-B. The Malate-acetyl-CoA-Glycerate (MCG) cycle aims to the production of acetyl-CoA with high efficiency coupled to carbon capture from bicarbonate anion and with recovery of metabolic intermediates produced during photorespiration. (H. Yu, X. Li, F. Duchoud, D. Chuang and J. Liao, "Augmenting the Calvin–Benson–Bassham cycle by a synthetic malyl-CoA-glycerate carbon fixation pathway", 2021).
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The part is flanked by two homology regions “    BBa_K3726112” 5CON1(H)_nNS5-up (PCC 11801) and “BBa_K3726113” 3CON5(H)_nNS5-down (PCC 11801) for the double homologous recombination within the genome of PCC 11801.
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Likewise, a Spectinomycin resistance cassette is included downstream the transcriptional unit region.
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Expression is controlled by the strong constitutive promoter P_PpsbA*-RiboJ “BBa_K3726079”  and the strong synthetic RBS, RBS_PtaBs  “BBa_K3726092”
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 00:40, 20 October 2021


Lv.1_PKMCG-A

This is a composite part that corresponds with a level 1 MoClo part in accordance with the marburg collection standard. It is a transcriptional unit for the expression of the part “BBa_K3726028” L0_PKMCG which contains the half part of the MCG cycle and the enzymes that belong to the phosphoketolase pathway (X. Liu, R. Miao, P. Lindberg and P. Lindblad, "Modular engineering for efficient photosynthetic biosynthesis of 1-butanol from CO2in cyanobacteria", 2021).

The other half of the MCG cycle is going to be expressed in the part MCGII_Lv1 which has two variants “BBa_K3726055” L1_MCGII-A and “BBa_K3726056” L1_MCGII-B. The Malate-acetyl-CoA-Glycerate (MCG) cycle aims to the production of acetyl-CoA with high efficiency coupled to carbon capture from bicarbonate anion and with recovery of metabolic intermediates produced during photorespiration. (H. Yu, X. Li, F. Duchoud, D. Chuang and J. Liao, "Augmenting the Calvin–Benson–Bassham cycle by a synthetic malyl-CoA-glycerate carbon fixation pathway", 2021).

The part is flanked by two homology regions “ BBa_K3726112” 5CON1(H)_nNS5-up (PCC 11801) and “BBa_K3726113” 3CON5(H)_nNS5-down (PCC 11801) for the double homologous recombination within the genome of PCC 11801.

Likewise, a Spectinomycin resistance cassette is included downstream the transcriptional unit region.

Expression is controlled by the strong constitutive promoter P_PpsbA*-RiboJ “BBa_K3726079” and the strong synthetic RBS, RBS_PtaBs “BBa_K3726092”


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 9525
    Illegal EcoRI site found at 1128
    Illegal SpeI site found at 10488
    Illegal PstI site found at 575
    Illegal PstI site found at 839
    Illegal PstI site found at 4411
    Illegal PstI site found at 5040
    Illegal PstI site found at 5537
    Illegal PstI site found at 6136
    Illegal PstI site found at 7074
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1128
    Illegal EcoRI site found at 9525
    Illegal NheI site found at 9177
    Illegal NheI site found at 9204
    Illegal SpeI site found at 10488
    Illegal PstI site found at 575
    Illegal PstI site found at 839
    Illegal PstI site found at 4411
    Illegal PstI site found at 5040
    Illegal PstI site found at 5537
    Illegal PstI site found at 6136
    Illegal PstI site found at 7074
    Illegal NotI site found at 9531
    Illegal NotI site found at 10495
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1128
    Illegal EcoRI site found at 9525
    Illegal BamHI site found at 505
    Illegal BamHI site found at 2950
    Illegal BamHI site found at 10070
    Illegal XhoI site found at 9401
    Illegal XhoI site found at 11519
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 9525
    Illegal suffix found in sequence at 10488
    Illegal EcoRI site found at 1128
    Illegal PstI site found at 575
    Illegal PstI site found at 839
    Illegal PstI site found at 4411
    Illegal PstI site found at 5040
    Illegal PstI site found at 5537
    Illegal PstI site found at 6136
    Illegal PstI site found at 7074
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 9525
    Illegal EcoRI site found at 1128
    Illegal XbaI site found at 9540
    Illegal SpeI site found at 10488
    Illegal PstI site found at 575
    Illegal PstI site found at 839
    Illegal PstI site found at 4411
    Illegal PstI site found at 5040
    Illegal PstI site found at 5537
    Illegal PstI site found at 6136
    Illegal PstI site found at 7074
    Illegal NgoMIV site found at 2527
    Illegal NgoMIV site found at 4636
    Illegal NgoMIV site found at 5096
    Illegal NgoMIV site found at 8992
    Illegal NgoMIV site found at 9134
    Illegal AgeI site found at 6649
    Illegal AgeI site found at 6732
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 9555
    Illegal BsaI.rc site found at 10472