Difference between revisions of "Part:BBa K3792004"

 
Line 1: Line 1:
−
 
 
__NOTOC__
 
__NOTOC__
−
<partinfo>BBa_K3792004 short</partinfo>
+
<partinfo>BBa_K3792003 short</partinfo>
 +
 
 +
apFAB51 is a strong promoter taken from the BIOFAB collection. This device uses apFAB51 to express mRFP1, a red fluorescent protein. The apFAB51 test device consists of apFAB51, an RBS, the coding sequence for mRFP1, and a terminator.
 +
 
 +
This test device and two others with BIOFAB promoters of different strengths were each assembled into the pSB1K3 plasmid and then transformed into E. coli NEB 5 alpha.
 +
 
 +
The fluorescence of the cells was measured using flow cytometry. By comparing the relative fluorescence, we can determine which promoters caused higher levels of expression of mRFP1.
 +
 
 +
 
  
−
The apFAB98 test device is a weak promotor that does not produce a lot of the protein mRFP1. The protein, mRFP1, is a red fluorescent protein.
 
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here
Line 10: Line 16:
 
<!-- -->
 
<!-- -->
 
<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
−
<partinfo>BBa_K3792004 SequenceAndFeatures</partinfo>
+
<partinfo>BBa_K3792003 SequenceAndFeatures</partinfo>
  
  
 
<!-- Uncomment this to enable Functional Parameter display  
 
<!-- Uncomment this to enable Functional Parameter display  
 
===Functional Parameters===
 
===Functional Parameters===
−
<partinfo>BBa_K3792004 parameters</partinfo>
+
<partinfo>BBa_K3792003 parameters</partinfo>
 
<!-- -->
 
<!-- -->

Revision as of 00:32, 20 October 2021

BIOFAB apFAB40 Measurement Device

apFAB51 is a strong promoter taken from the BIOFAB collection. This device uses apFAB51 to express mRFP1, a red fluorescent protein. The apFAB51 test device consists of apFAB51, an RBS, the coding sequence for mRFP1, and a terminator.

This test device and two others with BIOFAB promoters of different strengths were each assembled into the pSB1K3 plasmid and then transformed into E. coli NEB 5 alpha.

The fluorescence of the cells was measured using flow cytometry. By comparing the relative fluorescence, we can determine which promoters caused higher levels of expression of mRFP1.



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 614
    Illegal AgeI site found at 726
  • 1000
    COMPATIBLE WITH RFC[1000]