Difference between revisions of "Part:BBa K143036"

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<partinfo>BBa_K143036 short</partinfo>
 
<partinfo>BBa_K143036 short</partinfo>
  
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Transcription is regulated by proteins which bind operator sequences around the transcription start site. These proteins can positively affect transcription (activators) or negatively affect transcription (reppresors). Some repressor proteins can be inactivted however by addition of an inducer, such as xylose.
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Transcription is regulated by proteins which bind operator sequences around the transcription start site. These proteins can affect transcription positively (activators) or negatively (repressors). Many repressor proteins can be inactivated by addition of an inducer, such as xylose.
  
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XylR if the regulator protein for the Xylose operon in ''B. subtilis''<cite>#1</cite> and is responsible for ensuring that in the absence of xylose the xylose metabolism proteins are not expressed. Though endogenous to ''B. subtilis'', to minimise the leakage of a xylose inducible promoter XylR should be over-expressed. In the presence of xylose, the XylR tetramer is unable to bind DNA and so transcription resumes.  
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XylR is the regulatory protein of the Xylose operon in ''B. subtilis''<cite>#1</cite> and is responsible for ensuring the xylose metabolism proteins are not expressed in the absence of xylose . Though XylR is endogenous to ''B. subtilis'', '''XylR should be over-expressed''' to minimise the leakage of xylose inducible promoters. In the presence of xylose, the XylR multimer is unable to bind DNA and repression of transcription is released.  
  
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It must be noted that in all ''B. subtilis'' strains that do not have the Xylose operon knocked out '''the xylose inducer will gradually be metabolised by the host'''
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It must be noted that in all ''B. subtilis'' strains with a functional endoegouns xylose operon '''the xylose inducer will gradually be metabolised by the host'''.
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XylR was used in conjunction with the '''Xylose operon promoter''' (<bbpart>BBa_K143014<bbpart>) and acted as an input adaptor for a '''Polymerases per second''' (POPS) output
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XylR can be used in conjunction with the '''Xylose operon promoter''' (<bbpart>BBa_K143014</bbpart>), where the XylR will act as a reciever for an xylose input to result in an '''Polymerases per second''' (PoPS) output.
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<!-- Add more about the biology of this part here
 
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===Usage and Biology===
 
===Usage and Biology===
  
 
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<span class='h3bb'>Sequence and Features</span>
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<span class='h3bb'>'''<big>Sequence and Features</big>'''</span>
 
<partinfo>BBa_K143036 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K143036 SequenceAndFeatures</partinfo>
  
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<partinfo>BBa_K143036 parameters</partinfo>
 
<partinfo>BBa_K143036 parameters</partinfo>
 
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===References===
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<biblio>
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#1 pmid=2544559
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</biblio>
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==Modifications in Sequence : iGEM21_IISER-Tirupati_India==
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<partinfo>BBa_K3889092</partinfo> is a modified version specific to our project's assembly. Dual stop codons were removed for fusion with P2A linker (<partinfo>BBa_K3889069</partinfo>).

Latest revision as of 00:27, 20 October 2021

Xylose operon regulatory protein


Transcription is regulated by proteins which bind operator sequences around the transcription start site. These proteins can affect transcription positively (activators) or negatively (repressors). Many repressor proteins can be inactivated by addition of an inducer, such as xylose.

XylR is the regulatory protein of the Xylose operon in B. subtilis#1 and is responsible for ensuring the xylose metabolism proteins are not expressed in the absence of xylose . Though XylR is endogenous to B. subtilis, XylR should be over-expressed to minimise the leakage of xylose inducible promoters. In the presence of xylose, the XylR multimer is unable to bind DNA and repression of transcription is released.

It must be noted that in all B. subtilis strains with a functional endoegouns xylose operon the xylose inducer will gradually be metabolised by the host.

XylR can be used in conjunction with the Xylose operon promoter (BBa_K143014), where the XylR will act as a reciever for an xylose input to result in an Polymerases per second (PoPS) output.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


References

<biblio>

  1. 1 pmid=2544559

</biblio>


Modifications in Sequence : iGEM21_IISER-Tirupati_India

BBa_K3889092 is a modified version specific to our project's assembly. Dual stop codons were removed for fusion with P2A linker (BBa_K3889069).