Difference between revisions of "Part:BBa K3889020"
 
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P22 c2 repressor is from Salmonella P22 phage, which binds to its binding site (<partinfo>BBa_K3889080</partinfo> to <partinfo>BBa_K3889091</partinfo>) as a dimer leading to inhibition of the gene on which its binding site is present [1].
 
P22 c2 repressor is from Salmonella P22 phage, which binds to its binding site (<partinfo>BBa_K3889080</partinfo> to <partinfo>BBa_K3889091</partinfo>) as a dimer leading to inhibition of the gene on which its binding site is present [1].
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This is a modified version of <partinfo>BBa_C0053</partinfo> specific to our project's assembly.
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The following modifications were made:
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*Spa1 site Removed
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*LVA tag Removed
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*barcodes Removed
  
 
[[File:T--IISER-Tirupati India--P22 repression.png|centre]]
 
[[File:T--IISER-Tirupati India--P22 repression.png|centre]]
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===contributions===
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<!-- Add more about the biology of this part here
 
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Latest revision as of 00:17, 20 October 2021


P22 c2 repressor

P22 c2 repressor is from Salmonella P22 phage, which binds to its binding site (BBa_K3889080 to BBa_K3889091) as a dimer leading to inhibition of the gene on which its binding site is present [1].

This is a modified version of BBa_C0053 specific to our project's assembly. The following modifications were made:

  • Spa1 site Removed
  • LVA tag Removed
  • barcodes Removed
T--IISER-Tirupati India--P22 repression.png



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


References

1. Watkins, D., Hsiao, C., Woods, K. K., Koudelka, G. B., & Williams, L. D. (2008). P22 c2 Repressor−Operator Complex:  Mechanisms of Direct and Indirect Readout. Biochemistry, 47(8), 2325–2338. https://doi.org/10.1021/bi701826f