Difference between revisions of "Part:BBa K3889112"

Latest revision as of 23:02, 19 October 2021

Bacillus subtilis yqcF antitoxin cassette

This gene cassette has been designed to be a part of the genetic circuit for an inducible kill switch in Bacillus subtilis. The gene coding for the YqcF antitoxin (BBa_K3889093) is downstream to SP126 (BBa_K3889010) and RBS (BBa_K1351031) for constitutive expression. YqcF gene is linked with mCherry with the help of P2A Peptide Linker to check the working of the cassette in Bacillus subtilis.The cassette is complete with the double terminator (BBa_B0015).

The cassette has been designed to be a part of a genetic circuit of a xylose inducible kill switch. The constitutive expression of the YqcF antitoxin stabilises the system by reducing accidental death due to leaky expression of yqcG

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K3889112 short</partinfo>
-yqcF anti-toxin cassette for bacillus subtilis.
+This gene cassette has been designed to be a part of the genetic circuit for an inducible kill switch in <i>Bacillus subtilis</i>. The gene coding for the YqcF antitoxin (<partinfo>BBa_K3889093</partinfo>) is downstream to SP126 (<partinfo>BBa_K3889010</partinfo>) and RBS (<partinfo>BBa_K1351031</partinfo>) for constitutive expression. YqcF gene is linked with mCherry with the help of P2A Peptide Linker to check the working of the cassette in <i>Bacillus subtilis</i>.The cassette is complete with the double terminator (<partinfo>BBa_B0015</partinfo>).
+The cassette has been designed to be a part of a genetic circuit of a xylose inducible kill switch. The constitutive expression of the YqcF antitoxin stabilises the system by reducing accidental death due to leaky expression of yqcG
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