Difference between revisions of "Part:BBa K3886005"
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Since the Barcode needs to be subsequently detected by CRISPR-Cas12a based nucleic acids, we added the PAM sequence (TTTA) required for Cas12a at the 3' end of the Barcode. So the final sequence is "TTTACTCTACCTCGCTTCACGTCTGCTCACTCTGGTCCTAACAGCGATAGCGTCT" (Figure 1). | Since the Barcode needs to be subsequently detected by CRISPR-Cas12a based nucleic acids, we added the PAM sequence (TTTA) required for Cas12a at the 3' end of the Barcode. So the final sequence is "TTTACTCTACCTCGCTTCACGTCTGCTCACTCTGGTCCTAACAGCGATAGCGTCT" (Figure 1). | ||
</p> | </p> | ||
| − | <img src="https://2021.igem.org/wiki/images/9/9d/T--NDNF_China--part_barcode_table.png" alt="" width="400"> | + | <img align="center" src="https://2021.igem.org/wiki/images/9/9d/T--NDNF_China--part_barcode_table.png" alt="" width="400"> |
<h6 style="text-align:center">Figure 1: The code table for translating information into DNA barcode.</h6> | <h6 style="text-align:center">Figure 1: The code table for translating information into DNA barcode.</h6> | ||
Revision as of 22:47, 19 October 2021
NDNFCHINAiGEM2021 Barcode
This is a part used to encode "NDNFCHINAIGEM2021“ into DNA Sequence.
Characterization
In the Hidro system(for more information about Hidro, please visit: NDNF_China Description), we have incorporated a user-defined DNA barcode into engineered strains, which stores user-defined information to distinguish engineered bacteria from natural bacteria. This Barcode can effectively help researchers track possible escaped bacterial individuals and distinct engineered strains or natural ones in an out-of-lab environment.
To design the desired barcode, we used online tools to generate a DNA Barcode sequence to store "NDNFCHINAIGEM2021“ in the DNA sequence “CTCTACCTCGCTTCACGTCTGCTCACTCTGGTCCTAACAGCGATAGCGTCT”.
Since the Barcode needs to be subsequently detected by CRISPR-Cas12a based nucleic acids, we added the PAM sequence (TTTA) required for Cas12a at the 3' end of the Barcode. So the final sequence is "TTTACTCTACCTCGCTTCACGTCTGCTCACTCTGGTCCTAACAGCGATAGCGTCT" (Figure 1).
Figure 1: The code table for translating information into DNA barcode.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]