Difference between revisions of "Part:BBa K3886005"

Revision as of 22:45, 19 October 2021

NDNFCHINAiGEM2021 Barcode

This is a part used to encode "NDNFCHINAIGEM2021“ into DNA Sequence.

Characterization

In the Hidro system(for more information about Hidro, please visit: NDNF_China Description), we have incorporated a user-defined DNA barcode into engineered strains, which stores user-defined information to distinguish engineered bacteria from natural bacteria. This Barcode can effectively help researchers track possible escaped bacterial individuals and distinct engineered strains or natural ones in an out-of-lab environment.

To design the desired barcode, we used online tools to generate a DNA Barcode sequence to store "NDNFCHINAIGEM2021“ in the DNA sequence “CTCTACCTCGCTTCACGTCTGCTCACTCTGGTCCTAACAGCGATAGCGTCT”.

Since the Barcode needs to be subsequently detected by CRISPR-Cas12a based nucleic acids, we added the PAM sequence (TTTA) required for Cas12a at the 3' end of the Barcode. So the final sequence is "TTTACTCTACCTCGCTTCACGTCTGCTCACTCTGGTCCTAACAGCGATAGCGTCT" (Figure 1).

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 16: / Line 16: @@
 To design the desired barcode, we used <a href="https://earthsciweb.org/js/bio/dna-writer/">online tools</a> to generate a DNA Barcode sequence to store "NDNFCHINAIGEM2021“ in the DNA sequence “CTCTACCTCGCTTCACGTCTGCTCACTCTGGTCCTAACAGCGATAGCGTCT”.
 </p>
+<p>
+Since the Barcode needs to be subsequently detected by CRISPR-Cas12a based nucleic acids, we added the PAM sequence (TTTA) required for Cas12a at the 3' end of the Barcode. So the final sequence is "TTTACTCTACCTCGCTTCACGTCTGCTCACTCTGGTCCTAACAGCGATAGCGTCT" (Figure 1).
+</p>
+<img src="https://2021.igem.org/wiki/images/9/9d/T--NDNF_China--part_barcode_table.png" alt="" width="800">
 </html>