Difference between revisions of "Part:BBa K3726046:Design"

 
 
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__NOTOC__
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<partinfo>BBa_K3726046 short</partinfo>
  
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<partinfo>BBa_K3726046 SequenceAndFeatures</partinfo>
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===Design Notes===
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The part is a MoClo Lv.1 part, assembled in in the acceptor Lv1 entry vector whose ori is “BBa_K2560036”, and it's resistance cassette is “BBa_K3726039” (MARK_Lv0_AmpR).  This part has been assembled following the marburg collection standard, then the transcriptional unit is flanked by the part 5'Con3
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“BBa_K2560067” in its upstream region will allow the assembly of a Lv2 construct following the marburg collection standard. And by the part “BBa_K3726105” 3CON5(H)_NS1(mod)-down (PCC 11801) in its downstream region, which is a homology region for homologous recombination within the genome of PCC 11801.
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===Source===
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This construct has been made by golden gate reaction.
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===References===
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X. Liu, R. Miao, P. Lindberg and P. Lindblad, "Modular engineering for efficient photosynthetic biosynthesis of 1-butanol from CO2in cyanobacteria", 2021.

Latest revision as of 22:38, 19 October 2021

BOH1_C_Lv1


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1723
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 431
    Illegal NheI site found at 454
    Illegal NheI site found at 729
    Illegal NheI site found at 957
    Illegal PstI site found at 1723
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2057
    Illegal BglII site found at 2174
    Illegal BglII site found at 4593
    Illegal XhoI site found at 3277
    Illegal XhoI site found at 4120
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1723
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1723
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The part is a MoClo Lv.1 part, assembled in in the acceptor Lv1 entry vector whose ori is “BBa_K2560036”, and it's resistance cassette is “BBa_K3726039” (MARK_Lv0_AmpR). This part has been assembled following the marburg collection standard, then the transcriptional unit is flanked by the part 5'Con3 “BBa_K2560067” in its upstream region will allow the assembly of a Lv2 construct following the marburg collection standard. And by the part “BBa_K3726105” 3CON5(H)_NS1(mod)-down (PCC 11801) in its downstream region, which is a homology region for homologous recombination within the genome of PCC 11801.


Source

This construct has been made by golden gate reaction.

References

X. Liu, R. Miao, P. Lindberg and P. Lindblad, "Modular engineering for efficient photosynthetic biosynthesis of 1-butanol from CO2in cyanobacteria", 2021.