Difference between revisions of "Part:BBa M10025"

 
 
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__NOTOC__
 
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<partinfo>BBa_M10025 short</partinfo>
 
<partinfo>BBa_M10025 short</partinfo>
  
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TBA
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The purpose of this part is to encode a fast degrading GFP. Wildtype GFP typically have a half-life that may last for several bacterial generations. However, the addition of an AANDENYALAA tag to the C-terminal of the wildtype GFP will decrease this halflife to 40 minutes (Andersen et al, 1998). By changing the last three residues of this tag, various fast-degrading GFP were created. GFP[LVA] has a halflife of 40 minutes (Miller et al., 2000).
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Usage of a fast degrading GFP may act as a better reporter of changes in gene transcription over a period of time.
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PMID: 11059491
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----
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This part follows the BglBricks standard. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BglBricks Standard is available at: <br>
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[http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BglBricks Format]<br>
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 23:35, 10 May 2009

{˂GFP-LVA˃} Fast-Degrading GFP

The purpose of this part is to encode a fast degrading GFP. Wildtype GFP typically have a half-life that may last for several bacterial generations. However, the addition of an AANDENYALAA tag to the C-terminal of the wildtype GFP will decrease this halflife to 40 minutes (Andersen et al, 1998). By changing the last three residues of this tag, various fast-degrading GFP were created. GFP[LVA] has a halflife of 40 minutes (Miller et al., 2000).

Usage of a fast degrading GFP may act as a better reporter of changes in gene transcription over a period of time.

PMID: 11059491

This part follows the BglBricks standard. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BglBricks Standard is available at:
[http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BglBricks Format]

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 716
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 716
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 716
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 716
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 641