Difference between revisions of "Part:BBa K3771025"
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<br><b style="font-size:1.3rem">Description</b>
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<br><b style="font-size:1.3rem">Description</b><br>
 
<br>This composite part is a component of the IFN-γ sensing system and is used to express the taurine production enzyme, CSAD. <br>
 
<br>This composite part is a component of the IFN-γ sensing system and is used to express the taurine production enzyme, CSAD. <br>
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  <br><b style="font-size:1.3rem">Biology</b>
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  <br><b style="font-size:1.3rem">Biology</b><br>
 
<br>Binding of IFN-γ to the OmpA/OprF chimeric protein induces the response of the phage shock protein (Psp) system, a highly conserved stress response system in enterobacteria. <a href="https://pubmed.ncbi.nlm.nih.gov/16045608/" alt="" target="_blank">[3]</a> Signal transduction from the outer membrane to the inner membrane activates the pspA promoter, initiating expression of CSAD. CSAD catalyzes the decarboxylation of L-Cysteine sulfinic acid into hypotaurine, which is spontaneously oxidized to taurine <a href="https://pubmed.ncbi.nlm.nih.gov/30516051/" alt="" target="_blank">[4]</a>.  
 
<br>Binding of IFN-γ to the OmpA/OprF chimeric protein induces the response of the phage shock protein (Psp) system, a highly conserved stress response system in enterobacteria. <a href="https://pubmed.ncbi.nlm.nih.gov/16045608/" alt="" target="_blank">[3]</a> Signal transduction from the outer membrane to the inner membrane activates the pspA promoter, initiating expression of CSAD. CSAD catalyzes the decarboxylation of L-Cysteine sulfinic acid into hypotaurine, which is spontaneously oxidized to taurine <a href="https://pubmed.ncbi.nlm.nih.gov/30516051/" alt="" target="_blank">[4]</a>.  
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<div style="width=100%; display:flex; align-items: center; justify-content: center;">
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   <br><b style="font-size:1.3rem">Usage</b>
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<img src="https://2021.igem.org/wiki/images/c/c9/T--NCKU_Tainan--taurine_pathway_1.png" style="width:60%;">
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<p align="center">Fig.1 Taurine pathways in <i>E. coli</i></p>
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   <br><b style="font-size:1.3rem">Usage</b><br>
 
   <br>We ligased the <i>csad</i>-6xHis fragment and <i>pspA</i> promoter on the pSU expression vector and transformed it into DH5α to complete construction of the plasmid. The 6xHis allows for confirmation of CSAD expression by western blot using the anti-6xHis antibody. <br>
 
   <br>We ligased the <i>csad</i>-6xHis fragment and <i>pspA</i> promoter on the pSU expression vector and transformed it into DH5α to complete construction of the plasmid. The 6xHis allows for confirmation of CSAD expression by western blot using the anti-6xHis antibody. <br>
 
  <br><b style="font-size:1.3rem">Characterization</b>
 
  <br><b style="font-size:1.3rem">Characterization</b>
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<p align="center">圖片描述</p>
 
<p align="center">圖片描述</p>
  
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<br><b style="font-size:1.3rem">Reference</b>
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<br><b style="font-size:1.3rem">Reference</b><br>
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   <br>3. Darwin AJ. The phage-shock-protein response. Molecular Microbiology. 2005;57(3):621-628. doi:10.1111/j.1365-2958.2005.04694.x
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   <br>1. Darwin AJ. The phage-shock-protein response. Molecular Microbiology. 2005;57(3):621-628. doi:10.1111/j.1365-2958.2005.04694.x
 
<a href="https://pubmed.ncbi.nlm.nih.gov/16045608/
 
<a href="https://pubmed.ncbi.nlm.nih.gov/16045608/
 
" alt="" target="_blank">https://pubmed.ncbi.nlm.nih.gov/16045608/
 
" alt="" target="_blank">https://pubmed.ncbi.nlm.nih.gov/16045608/
 
</a><br>
 
</a><br>
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<br>4 Joo Y-C, Ko YJ, You SK, et al. Creating a New Pathway in Corynebacterium glutamicum for the Production of Taurine as a Food Additive. Journal of Agricultural and Food Chemistry. 2018;66(51):13454-13463. doi:10.1021/acs.jafc.8b05093
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<br>2. Joo Y-C, Ko YJ, You SK, et al. Creating a New Pathway in Corynebacterium glutamicum for the Production of Taurine as a Food Additive. Journal of Agricultural and Food Chemistry. 2018;66(51):13454-13463. doi:10.1021/acs.jafc.8b05093
 
<a href="https://pubmed.ncbi.nlm.nih.gov/30516051/
 
<a href="https://pubmed.ncbi.nlm.nih.gov/30516051/
 
" alt="" target="_blank">https://pubmed.ncbi.nlm.nih.gov/30516051/
 
" alt="" target="_blank">https://pubmed.ncbi.nlm.nih.gov/30516051/

Revision as of 20:33, 19 October 2021


PpspA-CSAD-6xHis


Description

This composite part is a component of the IFN-γ sensing system and is used to express the taurine production enzyme, CSAD.

Biology

Binding of IFN-γ to the OmpA/OprF chimeric protein induces the response of the phage shock protein (Psp) system, a highly conserved stress response system in enterobacteria. [3] Signal transduction from the outer membrane to the inner membrane activates the pspA promoter, initiating expression of CSAD. CSAD catalyzes the decarboxylation of L-Cysteine sulfinic acid into hypotaurine, which is spontaneously oxidized to taurine [4].

Fig.1 Taurine pathways in E. coli


Usage

We ligased the csad-6xHis fragment and pspA promoter on the pSU expression vector and transformed it into DH5α to complete construction of the plasmid. The 6xHis allows for confirmation of CSAD expression by western blot using the anti-6xHis antibody.

Characterization

圖片描述


Reference

1. Darwin AJ. The phage-shock-protein response. Molecular Microbiology. 2005;57(3):621-628. doi:10.1111/j.1365-2958.2005.04694.x https://pubmed.ncbi.nlm.nih.gov/16045608/

2. Joo Y-C, Ko YJ, You SK, et al. Creating a New Pathway in Corynebacterium glutamicum for the Production of Taurine as a Food Additive. Journal of Agricultural and Food Chemistry. 2018;66(51):13454-13463. doi:10.1021/acs.jafc.8b05093 https://pubmed.ncbi.nlm.nih.gov/30516051/
Sequence and Features BBa_K3771025 SequenceAndFeatures