Difference between revisions of "Part:BBa K3771022"
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  <br><b style="font-size:1.3rem">Biology</b><br>
 
  <br><b style="font-size:1.3rem">Biology</b><br>
 
   <br>Binding of IFN-γ to the OmpA/OprF chimeric protein induces the response of the phage shock protein (Psp) system, a highly conserved stress response system in enterobacteria. <a href="https://pubmed.ncbi.nlm.nih.gov/16045608/
 
   <br>Binding of IFN-γ to the OmpA/OprF chimeric protein induces the response of the phage shock protein (Psp) system, a highly conserved stress response system in enterobacteria. <a href="https://pubmed.ncbi.nlm.nih.gov/16045608/
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" alt="" target="_blank">[3]</a> Signal transduction from the outer membrane to the inner membrane activates the pspA promoter, initiating expression of CoaBC. CoaBC converts L-cystate into taurine in the taurine synthesis L-cystate pathway.
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" alt="" target="_blank">[1]</a> Signal transduction from the outer membrane to the inner membrane activates the pspA promoter, initiating expression of CoaBC. CoaBC converts L-cystate into taurine in the taurine synthesis L-cystate pathway.
 
<br>
 
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  <div style="width=100%; display:flex; align-items: center; justify-content: center;">
 
  <div style="width=100%; display:flex; align-items: center; justify-content: center;">
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</div>
 
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<p align="center">Fig.1 Taurine pathways in <i>E. coli</i></p>
 
<p align="center">Fig.1 Taurine pathways in <i>E. coli</i></p>
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   <br><b style="font-size:1.3rem">Usage</b>
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   <br><b style="font-size:1.3rem">Usage</b><br>
 
   <br>We ligased the <i>coaBC</i> fragment and <i>pspA</i> promoter on the pSU expression vector and transformed it into DH5α to complete construction of the plasmid. <br>
 
   <br>We ligased the <i>coaBC</i> fragment and <i>pspA</i> promoter on the pSU expression vector and transformed it into DH5α to complete construction of the plasmid. <br>
  
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<br><b style="font-size:1.3rem">Reference</b><br>
 
<br><b style="font-size:1.3rem">Reference</b><br>
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   <br>3. Darwin AJ. The phage-shock-protein response. Molecular Microbiology. 2005;57(3):621-628. doi:10.1111/j.1365-2958.2005.04694.x<a href="https://pubmed.ncbi.nlm.nih.gov/16045608/" alt="" target="_blank">https://pubmed.ncbi.nlm.nih.gov/16045608/</a><br>
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   <br>1. Darwin AJ. The phage-shock-protein response. Molecular Microbiology. 2005;57(3):621-628. doi:10.1111/j.1365-2958.2005.04694.x<a href="https://pubmed.ncbi.nlm.nih.gov/16045608/" alt="" target="_blank">https://pubmed.ncbi.nlm.nih.gov/16045608/</a><br>
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 20:19, 19 October 2021


PpspA-CoaBC


Description

This composite part is a component of the IFN-γ sensing system and was used to express the taurine production enzyme, CoaBC.

Biology

Binding of IFN-γ to the OmpA/OprF chimeric protein induces the response of the phage shock protein (Psp) system, a highly conserved stress response system in enterobacteria. [1] Signal transduction from the outer membrane to the inner membrane activates the pspA promoter, initiating expression of CoaBC. CoaBC converts L-cystate into taurine in the taurine synthesis L-cystate pathway.

Fig.1 Taurine pathways in E. coli


Usage

We ligased the coaBC fragment and pspA promoter on the pSU expression vector and transformed it into DH5α to complete construction of the plasmid.

Characterization

Fig. 2. Colony PCR confirmation of the construction


Reference

1. Darwin AJ. The phage-shock-protein response. Molecular Microbiology. 2005;57(3):621-628. doi:10.1111/j.1365-2958.2005.04694.xhttps://pubmed.ncbi.nlm.nih.gov/16045608/
Sequence and Features BBa_K3771022 SequenceAndFeatures